Szabó I, Renczes G, Gachályi B
Bethesda Children's Hospital, 2nd Department of Medicine, Budapest, Hungary.
Acta Physiol Hung. 1995;83(1):51-3.
Determination of the effect of the known enzyme-inducers phenobarbital-Na and spironolactone on the activity of the MFO was carried out. Male Wistar rats were treated either with 40 mg/kg phenobarbital-Na or 40 mg/kg spironolactone, and also in combination with 100 mg/kg of the MFO was inhibitor alpha-methyldopa. The activity of the MFO was assessed by changes in antipyrine half-life. Our results show that given alone both phenobarbital-Na and spironolactone are potent inducers of the MFO. Co-treatment with the enzyme-inhibitor alpha-methyldopa, however, led to different results in two cases: It slightly diminished the enzyme inducing effect of phenobarbital-Na, while the effect of spironolactone has completely ceased. These findings may suggest that phenobarbital-Na and spironolactone are acting on different cytochrome isoenzymes.
研究了已知的酶诱导剂苯巴比妥钠和螺内酯对混合功能氧化酶（MFO）活性的影响。雄性Wistar大鼠分别接受40mg/kg苯巴比妥钠或40mg/kg螺内酯治疗，同时还联合使用了100mg/kg的MFO抑制剂α-甲基多巴。通过安替比林半衰期的变化评估MFO的活性。我们的结果表明，单独给予苯巴比妥钠和螺内酯均是MFO的强效诱导剂。然而，与酶抑制剂α-甲基多巴联合治疗在两种情况下产生了不同的结果：它略微减弱了苯巴比妥钠的酶诱导作用，而螺内酯的作用则完全消失。这些发现可能表明苯巴比妥钠和螺内酯作用于不同的细胞色素同工酶。
