Hirasaka T
Department of Ophthalmology, Shinsuma Hospital, Hyogo-ken, Japan.
Nippon Ganka Gakkai Zasshi. 1995 Jul;99(7):770-7.
We investigated histologically wound healing after self-sealing cataract surgery, comparing cellular proliferation in scleral and corneal incisions. A scleral pocket (4 mm wide, 2 mm long) and internal corneal flap (2 mm long) were made in rabbit eyes on one side. After phacoemulsification, balanced salt solution was injected to self-seal the wound (scleral incision group). A corneal incision and internal corneal flap (4 mm wide, 2 mm long) were made in the contralateral eyes, and then the same procedure was performed (corneal incision group). On various days after operation, the eyes were analyzed by corneal topography and enucleated. The corneoscleral tissues were labelled with 3H-thymidine (37 degrees C, 4 hr) in Dulbecco's minimum essential medium, fixed with 4% paraformaldehyde, and subjected to autoradiography. On the first and fourth days after operation, the fibroblastic cells at the scleral side in the scleral incision group and keratocytes at the corneal side in the corneal incision group incorporated 3H-thymidine. On the seventh day after operation, the number of fibroblastic cells and 3H-thymidine uptake ratio were higher on the scleral side in the scleral incision group than on the corneal side in the corneal incision group. I suggest that the difference in the process of wound healing between the two groups might affect late postoperative astigmatism.
我们通过组织学方法研究了自密封白内障手术后的伤口愈合情况，比较了巩膜切口和角膜切口处的细胞增殖情况。在兔眼一侧制作一个巩膜袋（宽4毫米，长2毫米）和一个角膜内瓣（长2毫米）。超声乳化术后，注入平衡盐溶液以自密封伤口（巩膜切口组）。在对侧眼制作一个角膜切口和一个角膜内瓣（宽4毫米，长2毫米），然后进行相同操作（角膜切口组）。在术后不同天数，通过角膜地形图分析眼睛并摘除眼球。将角膜巩膜组织在杜尔贝科改良伊格尔培养基中用3H-胸腺嘧啶核苷标记（37℃，4小时），用4%多聚甲醛固定，然后进行放射自显影。术后第一天和第四天，巩膜切口组巩膜侧的成纤维细胞和角膜切口组角膜侧的角膜细胞摄取了3H-胸腺嘧啶核苷。术后第七天，巩膜切口组巩膜侧的成纤维细胞数量和3H-胸腺嘧啶核苷摄取率高于角膜切口组角膜侧。我认为两组伤口愈合过程的差异可能会影响术后晚期散光。
