Aronstein B N, Paterek J R, Kelley R L, Rice L E
Institute of Gas Technology, Des Plaines, IL 60018-1804, USA.
J Ind Microbiol. 1995 Jul;15(1):55-9. doi: 10.1007/BF01570014.
A series of experiments was conducted to examine the effects of chemical pretreatment on biodegradation of 14C-labeled PCB congeners in aqueous systems. Fenton's reagent was used to generate hydroxyl radicals (OH) which were successful in partially oxidizing/transforming otherwise recalcitrant molecules of tetrachlorinated PCB, but had little or no impact on the biodegradation of a monochlorinated congener. Application of Fenton's reagent (1% H2O2, 1 mM FeSO4) followed by inoculation with pure cultures Pseudomonas sp, strain LB 400 and Alcaligenes eutrophus, strain H850 resulted in the removal of approximately 38% of 2-chlorobiphenyl and 51% of 2,2',4,4'-tetrachlorobiphenyl in the form of 14CO2. Comparison of the rate and extent of biodegradation of 2,2',4,4'-tetrachlorobiphenyl after the application of Fenton's reagent with the dynamic and final level of radioactivity in the aqueous phase of experimental system suggests two possible means of microbial utilization of tetrachlorinated PCB congener altered by chemical oxidation: (a) consumption of the partially oxidized chemical dissolved in the aqueous phase, and (b) direct microbial attack on the transformed compound, which may still be adhered to the solid surface.
进行了一系列实验，以研究化学预处理对水体系中14C标记的多氯联苯同系物生物降解的影响。使用芬顿试剂产生羟基自由基（OH），该自由基成功地部分氧化/转化了四氯化多氯联苯原本难降解的分子，但对一氯化同系物的生物降解几乎没有影响。应用芬顿试剂（1% H2O2，1 mM FeSO4），然后接种纯培养物假单胞菌属菌株LB 400和嗜碱产碱菌菌株H850，导致以14CO2形式去除了约38%的2-氯联苯和51%的2,2',4,4'-四氯联苯。将芬顿试剂应用后2,2',4,4'-四氯联苯的生物降解速率和程度与实验系统水相中的动态和最终放射性水平进行比较，表明微生物利用经化学氧化改变的四氯化多氯联苯同系物有两种可能方式：（a）消耗溶解在水相中的部分氧化化学物质，以及（b）微生物直接攻击仍可能附着在固体表面的转化化合物。
