Department of Chemical Engineering, University of Texas, Austin TX 78712.
Biotechnol Bioeng. 1992 Dec 20;40(11):1395-402. doi: 10.1002/bit.260401114.
The white rot fungus Phanerochaete chrysosporium is unique in its ability to totally degrade a wide variety of recalcitrant pollutants. We have investigated the degradation of biphenyl and two model chlorinated biphenyls, 2,2',4,4'-tetrachlorobiphenyl and 2-chlorobiphenyl by suspended cultures of P. chrysosporium grown under conditions that maximize the synthesis of lignin-oxidizing enzymes. Radiolabeled biphenyl and 2'-chlorobiphenyl added to cultures at concentrations in the range 260 nM to 8.8 microM were degraded extensively to CO(2) within 30 days. In addition, from 40% to 60% of the recovered radioactivity was found in water-soluble compounds. A correlation between the rate of degradation and the synthesis of ligninases or Mn-dependent peroxidases could not be observed, indicating that yet unknown enzymatic system may be responsible for the initial oxidation of PCBs. The more heavily chlorinated PCB congener, 2,2',4,4'-tetrachlorobiphenyl was converted to CO(2) less readily; approximately 9% and 0.9% mineralization was observed in cultures incubated with 40 nM and 5.3 microM, respectively. Overall, our results indicate that P. chrysosporium is a promising organism for the treatment of wastes contaminated with lightly and moderately chlorinated PCBs.
白腐真菌糙皮侧耳（Phanerochaete chrysosporium）具有独特的能力，可以完全降解多种难降解的污染物。我们研究了悬浮培养的糙皮侧耳在最大程度合成木质素氧化酶的条件下对联苯和两种模型氯代联苯（2,2',4,4'-四氯联苯和 2-氯联苯）的降解。在 260 nM 至 8.8 microM 的浓度范围内添加到培养物中的放射性标记联苯和 2'-氯联苯在 30 天内被大量降解为 CO2。此外，从 40%到 60%的回收放射性物质存在于水溶性化合物中。降解速率与木质素酶或 Mn 依赖性过氧化物酶的合成之间没有观察到相关性，表明可能存在未知的酶系统负责 PCB 的初始氧化。更具氯化的 PCB 同系物 2,2',4,4'-四氯联苯不易转化为 CO2；在分别用 40 nM 和 5.3 microM 孵育的培养物中观察到约 9%和 0.9%的矿化。总的来说，我们的结果表明，糙皮侧耳是处理轻度和中度氯化的 PCB 污染废物的有前途的生物体。
