Qvist R, Morii H, Watanabe Y
Department of Neuroscience, Osaka Bioscience Institute, Japan.
Biochem Mol Biol Int. 1995 Feb;35(2):363-70.
Phospholipase A2 activities could be detected in both cytosolic and membrane fractions prepared from rat C6 glioma cells. The activities in both fractions were significantly increased by human recombinant interleukin-1 beta in a dose-dependent manner. Such a stimulation required a long-time exposure over 72 h and was partially inhibited by cycloheximide, a potent protein synthesis inhibitor. These results suggested that interleukin-1 beta may stimulate phospholipase A2 activity through its gene activation.
在从大鼠C6胶质瘤细胞制备的胞质溶胶和膜组分中均能检测到磷脂酶A2活性。人重组白细胞介素-1β以剂量依赖性方式显著增加了这两种组分中的活性。这种刺激需要超过72小时的长时间暴露，并且被强效蛋白质合成抑制剂环己酰亚胺部分抑制。这些结果表明白细胞介素-1β可能通过其基因激活来刺激磷脂酶A2活性。
