Postaire E, Regnault C, Simonet L, Rousset G, Bejot M
University Paris XI-Clinical Pharmacy, Châtenay-Malabry, France.
Biochem Mol Biol Int. 1995 Feb;35(2):371-4.
Substantial evidence supports the theory that free radicals, especially oxygen radicals, are involved in the process of aging. The human organisms have two ways to fight them: an enzymatic way with enzymatic intervention like superoxide dismutase, catalase... and a chemical way with the intervention of scavengers such as vitamins, cysteine, methionine, gluthatione... The aim of this work was to determine that an intakes of vitamins association: vitamin E, vitamin C and beta carotene induce an increase of singlet oxygen protection of erythrocytes' subjects. The method was based on the haemolytic effect of singlet oxygen which is generated by irradiation of hematoporphyrine at 365 nm, in 22 p. cent suspension of erythrocytes' subjects. Results show that a supply of beta carotene (15 or 30 mg/day), vitamin E (15 mg/day) and vitamin C (30 mg/day) involves an increase of singlet oxygen protection of erythrocytes of subjects. This protection appears very quickly after 15 days of treatment.
大量证据支持自由基，尤其是氧自由基参与衰老过程的理论。人类机体有两种对抗自由基的方式：一种是酶促方式，通过超氧化物歧化酶、过氧化氢酶等酶的干预；另一种是化学方式，通过维生素、半胱氨酸、蛋氨酸、谷胱甘肽等清除剂的干预。这项工作的目的是确定维生素E、维生素C和β-胡萝卜素的联合摄入会使红细胞受试者对单线态氧的保护作用增强。该方法基于在22%红细胞受试者悬浮液中，通过365nm波长照射血卟啉产生的单线态氧的溶血效应。结果表明，补充β-胡萝卜素（15或30毫克/天）、维生素E（15毫克/天）和维生素C（30毫克/天）会使受试者红细胞对单线态氧的保护作用增强。这种保护作用在治疗15天后很快就会出现。
