Regnault C, Postaire E R, Rousset G J, Bejot M, Hazebroucq G F
Department of Clinical Pharmacy, School of Pharmacy, Châtenay-Malabry, France.
Ann Pharmacother. 1993 Nov;27(11):1349-50. doi: 10.1177/106002809302701108.
To evaluate the in vivo radical scavenger activity of vitamin E, vitamin C, and beta carotene on erythrocyte membranes.
A prospective, open trial without placebo.
Department of Clinical Pharmacy.
Ten healthy volunteers being supplemented with beta carotene, vitamin E, and vitamin C.
Erythrocytes were incubated in water bath with 2,2' azobis (2 amidinopropane) hydrochloride (AAPH). AAPH decomposes spontaneously at 37 degrees C to generate free radicals inducing membrane cellular damage and hemolysis. The absorbance was measured at 405 nm at 0, 30, and 60 min, and then every 20 minutes for four hours. The time for 50 percent of maximal hemolysis (T50%), which expresses the radical scavenger activity of erythrocytes, was determined.
The physiologic T50% value determined in 52 healthy volunteers is 117 +/- 12 min. Patients receiving these supplements have a higher value of T50% (143.2 +/- 11.6 min at 30 d and 145.7 +/- 10.5 min at 60 d) than the physiologic value (p < 0.001).
These data suggest that vitamin C, vitamin E, and beta carotene stimulate the radical scavenger activity of erythrocyte membranes after 30 days.
评估维生素E、维生素C和β-胡萝卜素对红细胞膜的体内自由基清除活性。
一项无安慰剂的前瞻性开放试验。
临床药学系。
十名补充β-胡萝卜素、维生素E和维生素C的健康志愿者。
将红细胞与2,2'-偶氮二（2-脒基丙烷）盐酸盐（AAPH）在水浴中孵育。AAPH在37℃下自发分解产生自由基，诱导膜细胞损伤和溶血。在0、30和60分钟时于405nm处测量吸光度，然后每20分钟测量一次，共四小时。确定达到最大溶血50%的时间（T50%），该时间表示红细胞的自由基清除活性。
在52名健康志愿者中测定的生理T50%值为117±12分钟。接受这些补充剂的患者的T50%值（30天时为143.2±11.6分钟，60天时为145.7±10.5分钟）高于生理值（p<0.001）。
这些数据表明，30天后维生素C、维生素E和β-胡萝卜素可刺激红细胞膜的自由基清除活性。
