Yang C S, Tsai P J, Chen W Y, Liu L, Kuo J S
Department of Medical Research, Taichung Veterans General Hospital, Taiwan.
J Chromatogr B Biomed Appl. 1995 May 5;667(1):41-8. doi: 10.1016/0378-4347(94)00590-2.
An on-line analytical system for the continuous in vivo monitoring of extracellular glutathione (gamma-glutamyl-cysteinylglycine) (GSH) concentrations in the livers of anaesthetized rats was developed. Microdialysates perfused through implanted microdialysis probes were collected with a loading loop of an on-line injector for direct and automatic injection into a high-performance liquid chromatographic system equipped with an electrochemical detector with Au-Hg electrodes. This method shortened the analysis time and circumvented the sample preparation process which is essential for accurate determination of GSH levels in biological samples. Additionally, this method provided continuous and real-time monitoring of extracellular GSH levels. Basal extracellular GSH concentrations in the livers of anaesthetized rate were found to vary over a wide range (from 4.16 to 76.5 microM). The method was applied to study the effect of global liver ischaemia on extracellular GSH concentrations and it was found that extracellular GSH levels in livers increased immediately with the onset of ischaemia and remained elevated for the 30-min ischaemic period. Ensuing reperfusion did reduce the GSH increase; however, the GSH levels did not return to the basal value.
开发了一种在线分析系统，用于连续体内监测麻醉大鼠肝脏细胞外谷胱甘肽（γ-谷氨酰-半胱氨酰甘氨酸）（GSH）浓度。通过植入的微透析探针灌注的微透析液，用在线进样器的进样环收集，以便直接自动注入配备有金汞电极的电化学检测器的高效液相色谱系统。该方法缩短了分析时间，规避了生物样品中GSH水平准确测定所必需的样品制备过程。此外，该方法可对细胞外GSH水平进行连续实时监测。发现麻醉大鼠肝脏的基础细胞外GSH浓度在很宽的范围内变化（从4.16到76.5微摩尔）。该方法用于研究全肝缺血对细胞外GSH浓度的影响，结果发现肝脏细胞外GSH水平在缺血开始时立即升高，并在30分钟的缺血期内保持升高。随后的再灌注确实降低了GSH的升高；然而，GSH水平并未恢复到基础值。
