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与传统和微量分析系统相结合用于监测生物事件的微透析采样的最新趋势：综述

Recent trends in microdialysis sampling integrated with conventional and microanalytical systems for monitoring biological events: a review.

作者信息

Nandi Pradyot, Lunte Susan M

机构信息

Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66047, United States.

出版信息

Anal Chim Acta. 2009 Sep 28;651(1):1-14. doi: 10.1016/j.aca.2009.07.064. Epub 2009 Aug 3.

DOI:10.1016/j.aca.2009.07.064
PMID:19733728
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3744124/
Abstract

Microdialysis (MD) is a sampling technique that can be employed to monitor biological events both in vivo and in vitro. When it is coupled to an analytical system, microdialysis can provide near real-time information on the time-dependent concentration changes of analytes in the extracellular space or other aqueous environments. Online systems for the analysis of microdialysis samples enable fast, selective and sensitive analysis while preserving the temporal information. Analytical methods employed for online analysis include liquid chromatography (LC), capillary (CE) and microchip electrophoresis and flow-through biosensor devices. This review article provides an overview of microdialysis sampling and online analysis systems with emphasis on in vivo analysis. Factors that affect the frequency of analysis and, hence, the temporal resolution of these systems are also discussed.

摘要

微透析（MD）是一种可用于监测体内和体外生物事件的采样技术。当它与分析系统耦合时，微透析可以提供细胞外空间或其他水性环境中分析物随时间变化的浓度的近实时信息。用于分析微透析样品的在线系统能够在保留时间信息的同时实现快速、选择性和灵敏的分析。用于在线分析的分析方法包括液相色谱（LC）、毛细管电泳（CE）、芯片电泳和流通式生物传感器设备。这篇综述文章概述了微透析采样和在线分析系统，重点是体内分析。还讨论了影响分析频率以及这些系统时间分辨率的因素。

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