采用顶空气相色谱法测定人和动物血液中的氯氟烃替代物1,1,1,2 - 四氟乙烷（HFA - 134a）。

Determination of the chlorofluorocarbon substitute 1,1,1,2-tetrafluoroethane (HFA-134a) in human and animal blood using gas chromatography with headspace analysis.

作者信息

Cooper K M, Chang S F, Harrison L I

机构信息

Department of Drug Metabolism, 3M Pharmaceuticals, St. Paul, MN 55144-1000, USA.

出版信息

J Chromatogr B Biomed Appl. 1995 May 5;667(1):85-93. doi: 10.1016/0378-4347(95)00013-9.

DOI:10.1016/0378-4347(95)00013-9

PMID:7663689

Abstract

A gas chromatographic procedure with headspace analysis and flame-ionization detection is described for the determination of the chlorofluorocarbon substitute 1,1,1,2-tetrafluoroethane (HFA-134a). A 0.5-2 ml sample of heparinized whole blood from a laboratory animal or human is added directly into a presealed headspace vial from which an equivalent volume of air has been removed. The internal standard 1,1,2,2-tetrafluoroethane is added and the sample frozen until analysis. Chromatographic separation is achieved using a PoraPlot Q porous-layer capillary column. The analytical range is 5.8-3298 ng/ml when 2-ml human blood samples are used. The concentration range of the calibration curve can be easily adapted to accommodate the concentrations expected in either pharmacokinetic or toxicokinetic studies. Automation of the assay permits the maximum number of samples to be processed in a day.

摘要

描述了一种采用顶空分析和火焰离子化检测的气相色谱法，用于测定氯氟烃替代物1,1,1,2 - 四氟乙烷（HFA - 134a）。将0.5 - 2 ml来自实验动物或人类的肝素化全血样本直接加入到一个预先密封的顶空瓶中，该顶空瓶中已去除等量体积的空气。加入内标物1,1,2,2 - 四氟乙烷，将样品冷冻直至分析。使用PoraPlot Q多孔层毛细管柱实现色谱分离。当使用2 ml人体血液样本时，分析范围为5.8 - 3298 ng/ml。校准曲线的浓度范围可轻松调整，以适应药代动力学或毒代动力学研究中预期的浓度。该测定方法的自动化使得一天内能够处理的样品数量达到最多。