De Barro P J, Sherratt T N, Carvalho G R, Nicol D, Iyengar A, MacLean N
Department of Biology, University of Southampton, UK.
Mol Ecol. 1995 Jun;4(3):375-82. doi: 10.1111/j.1365-294x.1995.tb00230.x.
Samples of the grain aphid Sitobion avenae (F.) and the rose-grain aphid Metopolophium dirhodum (Walker) were collected in late March from wheat fields and adjacent road-side grasses at a number of locations in southern England. Unparasitized aphids were DNA fingerprinted using the multilocus (GATA)4 probe. Over all locations, the fingerprints of individual S. avenae caught in wheat had lower overall average distances of band migration (ADBM) and shared a higher proportion of bands, than fingerprints of individuals caught in adjacent road-side grasses. The ADBM of fingerprints of S. avenae collected on road-side grasses altered significantly with geographical location, while the ADBM of fingerprints of S. avenae caught on wheat did not. A comparison of the fingerprints of individual M. dirhodum caught in wheat and neighbouring road-side grasses did not reveal any genetic differentiation. Fingerprints of M. dirhodum that were caught in the same host type did however, show significant variation in ADBM between different locations. With both S. avenae and M. dirhodum, spatial autocorrelation revealed that locations that were close together were no more likely to have individuals with similar ADBM than locations that were far apart. Our results suggest that (i) particular clones of S. avenae prefer to colonize wheat, and/or that (ii) particular clones of S. avenae perform better on wheat than other clones. It is unclear why M. dirhodum did not show any genetic structuring according to host type, but this species appears to engage in sexual reproduction much more frequently than S. avenae in southern England.(ABSTRACT TRUNCATED AT 250 WORDS)
3月下旬，在英格兰南部的多个地点，从小麦田和相邻路边草丛中采集了麦长管蚜（Sitobion avenae (F.)）和禾谷缢管蚜（Metopolophium dirhodum (Walker)）样本。使用多位点（GATA）4探针，对未被寄生的蚜虫进行DNA指纹分析。在所有地点，在小麦中捕获的单个麦长管蚜的指纹，其条带迁移的总体平均距离（ADBM）较低，并且条带共享比例更高，这比在相邻路边草丛中捕获的个体的指纹情况要好。在路边草丛中采集的麦长管蚜指纹的ADBM随地理位置有显著变化，而在小麦上捕获的麦长管蚜指纹的ADBM则没有这种变化。对在小麦和相邻路边草丛中捕获的单个禾谷缢管蚜的指纹进行比较，未发现任何遗传分化。然而，在相同寄主类型中捕获的禾谷缢管蚜指纹，在不同地点之间的ADBM显示出显著差异。对于麦长管蚜和禾谷缢管蚜，空间自相关分析表明，距离较近的地点并不比距离较远的地点更有可能拥有具有相似ADBM的个体。我们的结果表明：（i）麦长管蚜的特定克隆更倾向于在小麦上定殖，和/或（ii）麦长管蚜的特定克隆在小麦上的表现优于其他克隆。目前尚不清楚为什么禾谷缢管蚜没有根据寄主类型表现出任何遗传结构，但在英格兰南部，该物种似乎比麦长管蚜更频繁地进行有性繁殖。（摘要截选至250字）
