De Barro P J, Sherratt T N, Carvalho G R, Nicol D, Iyengar A, Maclean N
Department of Biology, University of Southampton.
Insect Mol Biol. 1994 Nov;3(4):253-60. doi: 10.1111/j.1365-2583.1994.tb00174.x.
Grain aphids (Sitobion avenae (F.)) were collected from forty-four wheat ears in a Hampshire field at three times during the growing season. On each occasion, individual aphids were profiled using the multilocus (GATA)4 probe. During the full head emergence and full anthesis growth stages of wheat, each ear generally supported a genetically distinguishable aphid colony which consisted of genetically indistinguishable individuals (putative clones). This information strongly suggests that individual ears were colonized by single immigration events. By the late milky ripe stage, most ears supported two or more such clones. The total number of clones declined and the spatial separation of identical clones increased markedly over the duration of the study, which strongly suggests that secondary spread rather than increased immigration was responsible for the increased clonal diversity of ears. In addition, the profiles of individual S. avenae became more alike as the season progressed and samples became dominated by particular clones indicating either differential survival or reproduction among clones.
在生长季节，分三次从汉普郡一块田地的44个麦穗上采集麦长管蚜（Sitobion avenae (F.)）。每次采集时，都使用多位点（GATA）4探针分析单个蚜虫的基因特征。在小麦全穗抽出期和完全开花期，每个麦穗通常都有一个基因上可区分的蚜虫群体，该群体由基因上无法区分的个体（假定的克隆体）组成。这一信息有力地表明，单个麦穗是由单次迁入事件定殖的。到乳熟后期，大多数麦穗上有两个或更多这样的克隆体。在研究期间，克隆体的总数下降，相同克隆体的空间分离显著增加，这有力地表明，麦穗上克隆多样性的增加是由二次传播而非迁入增加所致。此外，随着季节的推进，单个麦长管蚜的基因特征变得更加相似，样本中特定克隆体占主导地位，这表明克隆体之间存在不同的存活率或繁殖率。
