Allen G V, Zhou J, Hopkins D A
Department of Anatomy and Neurobiology, Faculty of Medicine, Dalhousie University, Halifax, Canada.
Brain Res. 1995 May 22;680(1-2):117-27. doi: 10.1016/0006-8993(95)00252-l.
The development of dystrophic cardiac muscle is related to increases in sympathetic nervous system activity but little is known regarding possible central neural mechanisms that may be involved in cardiomyopathy. The inbred cardiomyopathic hamster is an animal model for studying the development and mechanisms of necrosis in cardiac muscle which resemble non-vascular myocardial diseases of man. Because monoamines are known to play a major role in central regulation of the cardiovascular system, we compared the distribution and density of tyrosine hydroxylase (TH) and 5-hydroxytryptamine (5-HT) immunostaining in the brains of cardiomyopathic hamsters (strain CHF-146), a related strain (CHF-148) of non-cardiomyopathic albino hamsters, and golden Syrian hamsters for possible differences in neurochemical organization. At the time of sacrifice, the cardiomyopathic hamsters exhibit small, calcified lesions on the surface of the ventricular cardiac muscle (early necrotic phase). Brain sections from each group were processed identically and simultaneously. The results show that there were significant differences among strains in the parabrachial nucleus with respect to the two neurochemicals examined. In golden Syrian and albino hamsters, TH and 5-HT immunoreactive axons were lightly-to-moderately stained in the lateral parabrachial nucleus. In the cardiomyopathic hamster, there were significantly more densely stained TH and 5-HT immunoreactive axons in the lateral parabrachial nucleus, in particular the inner part of the external lateral subnucleus. Because the lateral parabrachial nucleus, including the external lateral subnucleus, is known to be involved in regulation of the cardiovascular system, the differential distribution of TH and 5-HT in the parabrachial nucleus of cardiomyopathic hamsters in comparison to normal hamsters suggests that the parabrachial nucleus could be involved in sympathetic mechanisms related to the development of necrosis in cardiac muscle of the cardiomyopathic hamster.
营养不良性心肌的发展与交感神经系统活动的增加有关，但关于心肌病可能涉及的中枢神经机制却知之甚少。近交系心肌病仓鼠是一种动物模型，用于研究与人类非血管性心肌疾病相似的心肌坏死的发展和机制。由于已知单胺在心血管系统的中枢调节中起主要作用，我们比较了心肌病仓鼠（CHF - 146品系）、非心肌病白化仓鼠的相关品系（CHF - 148）和金黄叙利亚仓鼠大脑中酪氨酸羟化酶（TH）和5 - 羟色胺（5 - HT）免疫染色的分布和密度，以寻找神经化学组织方面可能存在的差异。在处死时，心肌病仓鼠的心室心肌表面出现小的钙化病变（早期坏死阶段）。每组的脑切片以相同且同时的方式进行处理。结果表明，在所检查的两种神经化学物质方面，臂旁核在各品系之间存在显著差异。在金黄叙利亚仓鼠和白化仓鼠中，TH和5 - HT免疫反应性轴突在外侧臂旁核中呈轻度至中度染色。在心肌病仓鼠中，外侧臂旁核，特别是外侧外侧亚核的内部，有明显更多的TH和5 - HT免疫反应性轴突密集染色。由于已知包括外侧外侧亚核在内的外侧臂旁核参与心血管系统的调节，与正常仓鼠相比，心肌病仓鼠臂旁核中TH和5 - HT的差异分布表明，臂旁核可能参与了与心肌病仓鼠心肌坏死发展相关的交感神经机制。
