Sauter G, Moch H, Wagner U, Novotna H, Gasser T C, Mattarelli G, Mihatsch M J, Waldman F M
Institute of Pathology, University of Basel, Switzerland.
Cancer Genet Cytogenet. 1995 Jul 15;82(2):163-9. doi: 10.1016/0165-4608(95)00030-s.
To examine the significance of Y chromosome losses in bladder cancer, fluorescence in situ hybridization (FISH) was used to determine its prevalence and associations with known parameters of malignancy. Cells were dissociated from formalin-fixed paraffin-embedded bladder tumors from 68 male patients and from 11 post-mortem bladder washes of male patients with a negative bladder cancer history, and were examined by FISH using centromeric probes for chromosomes X, Y, 7, 9, and 17. Nullisomy for chromosome Y was seen in 23 of 68 tumors (34%), monosomy in 28 of 68 tumors (41%), and polysomy in 17 of 68 tumors (25%). There was no association between chromosome Y loss and tumor grade, stage, tumor growth fraction (Ki67 LI), p53 immunostaining, and presence of p53 deletions. Patient age was higher for tumors with a Y loss (73.5 +/- 12.0 years) than for tumors without Y loss (66.6 +/- 10.8 years; p = 0.0207). In one normal bladder wash, a distinct subpopulation (38% of cells) with Y nullisomy was seen. These data suggest that Y loss is a frequent event that can occur early in bladder cancer, although there is no evidence for a role of Y loss in tumor progression.
为了研究Y染色体缺失在膀胱癌中的意义，采用荧光原位杂交（FISH）技术来确定其发生率以及与已知恶性参数的相关性。从68例男性患者的福尔马林固定石蜡包埋膀胱肿瘤中以及11例有阴性膀胱癌病史男性患者的尸检膀胱冲洗液中分离细胞，并使用针对X、Y、7、9和17号染色体的着丝粒探针通过FISH进行检测。在68例肿瘤中有23例（34%）出现Y染色体缺体，28例（41%）出现单体，17例（25%）出现多体。Y染色体缺失与肿瘤分级、分期、肿瘤生长分数（Ki67 LI）、p53免疫染色以及p53缺失的存在均无相关性。Y染色体缺失的肿瘤患者年龄（73.5±12.0岁）高于无Y染色体缺失的肿瘤患者（66.6±10.8岁；p = 0.0207）。在一次正常膀胱冲洗液中，发现了一个具有Y染色体缺体的明显亚群（占细胞的38%）。这些数据表明，Y染色体缺失是一种常见现象，可在膀胱癌早期发生，尽管没有证据表明Y染色体缺失在肿瘤进展中起作用。
