Hansen S K, Tjian R
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.
Cell. 1995 Aug 25;82(4):565-75. doi: 10.1016/0092-8674(95)90029-2.
The D. melanogaster alcohol dehydrogenase (Adh) gene is transcribed from two tandem promoters that are differentially utilized at various stages during development. To determine the mechanism of promoter selectivity, we have analyzed the activity of the Adh promoters both in vitro and in transfected cells. We found that selective promoter utilization is controlled by distinct initiator elements. Reconstitution of Adh transcription with purified components requires a specific TBP-TAF complex that, in concert with TFIIA, directs differential Adh promoter transcription. Fractionation of this TBP-TAF complex reveals that TAFII150 is required for discrimination between the proximal and distal promoters. We propose a mechanism for regulating differential promoter utilization during Drosophila development that involves the recognition of specific initiator elements by TAFs in the TFIID complex.
黑腹果蝇乙醇脱氢酶（Adh）基因由两个串联启动子转录，这两个启动子在发育的不同阶段被差异利用。为了确定启动子选择性的机制，我们在体外和转染细胞中分析了Adh启动子的活性。我们发现选择性启动子利用受不同的起始元件控制。用纯化成分重建Adh转录需要一种特定的TBP-TAF复合物，该复合物与TFIIA协同作用，指导Adh启动子的差异转录。对这种TBP-TAF复合物的分级分离表明，TAFII150是区分近端和远端启动子所必需的。我们提出了一种在果蝇发育过程中调节启动子差异利用的机制，该机制涉及TFIID复合物中的TAF对特定起始元件的识别。
