Zhang W, Voice J, Lachmann P J
Molecular Immunopathology Unit, MRC Centre, Cambridge, UK.
Clin Exp Immunol. 1995 Sep;101(3):507-14. doi: 10.1111/j.1365-2249.1995.tb03142.x.
Defined immune complexes (IC) were used to compare the effect of antibodies of different classes and subclasses on neutrophil respiratory burst and degranulation. IC were made from 5-iodo-4-hydroxy-3-nitrophenacetyl (NIP) conjugated to bovine serum albumin (BSA) and chimaeric mouse-human anti-NIP monoclonal antibodies including IgA2, IgE and all four IgG subclasses. The activation of neutrophils by IC depended on antibody class and subclass, on antigen epitope density, on antigen: antibody ratio and on the medium used. The ability to generate the respiratory burst showed a different pattern to the ability to give rise to degranulation. Compared with other IC, IgA2 IC provided the strongest stimulus for neutrophil activation. IgG1 IC, IgG2 IC and IgG4 IC activated neutrophils moderately or weakly IgG3 IC were unable to stimulate the respiratory burst, but could cause strong degranulation. IgE IC could hardly cause any neutrophil response. Neutrophil degranulation in response to IgG3 IC in serum-free medium or heat-inactivated serum was fast, and it quickly reached maximum. Degranulation caused by IgA IC was relatively slow, but gradually increased during incubation. The activity of IgG1 IC, IgG2 IC and IgG4 IC generated a respiratory burst increased with antibody excess and decreased with antigen excess. The activity of IgA2 IC, however, was not affected by change of antigen and antibody ratio. A specific role of serum, possibly due to complement, was found in enhancing degranulation, both temporally and quantitatively, by IgA2 IC.
使用特定的免疫复合物（IC）来比较不同类别和亚类抗体对中性粒细胞呼吸爆发和脱颗粒的影响。IC由与牛血清白蛋白（BSA）偶联的5-碘-4-羟基-3-硝基苯乙酰（NIP）以及嵌合的小鼠-人抗NIP单克隆抗体组成，包括IgA2、IgE和所有四种IgG亚类。IC对中性粒细胞的激活取决于抗体的类别和亚类、抗原表位密度、抗原与抗体的比例以及所用的培养基。产生呼吸爆发的能力与引起脱颗粒的能力呈现出不同的模式。与其他IC相比，IgA2 IC对中性粒细胞激活的刺激最强。IgG1 IC、IgG2 IC和IgG4 IC对中性粒细胞的激活程度中等或较弱，IgG3 IC无法刺激呼吸爆发，但可引起强烈的脱颗粒。IgE IC几乎不会引起任何中性粒细胞反应。在无血清培养基或热灭活血清中，中性粒细胞对IgG3 IC的脱颗粒反应迅速，且很快达到最大值。IgA IC引起的脱颗粒相对较慢，但在孵育过程中逐渐增加。IgG1 IC、IgG2 IC和IgG4 IC产生呼吸爆发的活性随抗体过量而增加，随抗原过量而降低。然而，IgA2 IC的活性不受抗原与抗体比例变化的影响。发现血清可能通过补体在增强IgA2 IC引起的脱颗粒方面具有特定作用，在时间和数量上均有体现。
