Li S, Ding Z, Liang Y
People's Hospital of Panyu City, Guangdong.
Zhonghua Fu Chan Ke Za Zhi. 1995 Apr;30(4):200-2.
For those pregnant women with an abnormal pregnancy history, the polymerase chain reaction (PCR) technique was adopted to screening the serum toxoplasmosis DNA (TOX-DNA). Then tests were made for further evidence of TOX-DNA in the amnionic fluids TOX-DNA was examined in cases with blood TOX-DNA positive. Gamma glutamyl transpeptidase (GGT) and alpha-fetoprotein (AFP) in the amnionic fluids were measured for reference, a antenatal diagnosis of the fetal congenital toxoplasmosis infection could thus be made. In the present paper, 9 out of 92 blood specimen were TOX-DNA positive, of the 9 cases, 5 had TOX-DNA and 4 had no TOX-DNA in the amnionic fluid tests. The results of follow-up examination in the 9 cases were in conformity with the ante natal diagnosis. This is an important procedure for diagnosis and prevention of maternal-fetal vertical infection of Toxoplasmosis.
对于有异常妊娠史的孕妇，采用聚合酶链反应（PCR）技术筛查血清弓形虫病DNA（TOX-DNA）。然后对羊水进行检测以进一步证实血液TOX-DNA阳性病例中的TOX-DNA。检测羊水中的γ-谷氨酰转肽酶（GGT）和甲胎蛋白（AFP）作为参考，从而对胎儿先天性弓形虫感染进行产前诊断。在本文中，92份血液标本中有9份TOX-DNA呈阳性，在这9例中，羊水检测有5例存在TOX-DNA，4例不存在TOX-DNA。9例的随访检查结果与产前诊断一致。这是诊断和预防母婴垂直感染弓形虫病的重要步骤。
