人类血型Sd(a+)和Sd(a-)的Tamm-Horsfall糖蛋白与双花扁豆和绒毛野豌豆-B4凝集素的差异结合

Differential binding of human blood group Sd(a+) and Sd(a-) Tamm-Horsfall glycoproteins with Dolichos biflorus and Vicia villosa-B4 agglutinins.

作者信息

Wu A M, Wu J H, Watkins W M, Chen C P, Song S C, Chen Y Y

机构信息

Glycoimmunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang-Gung Medical College, Kwei-san, Tao-yuan, Taiwan.

出版信息

FEBS Lett. 1998 Jun 16;429(3):323-6. doi: 10.1016/s0014-5793(98)00617-6.

DOI:10.1016/s0014-5793(98)00617-6

PMID:9662441

Abstract

The binding patterns of human blood group Sd(a+) and Sd(a-) Tamm-Horsfall glycoproteins (THGPs) with respect to four GalNAc specific agglutinins were studied by quantitative precipitin assay (QPA) and enzyme linked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a-) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bound well with Dolichos biflorus (DBA) and Vicia villosa-B4 (VVA-B4), while Sd(a-) THGP reacted poorly with these two lectins. Neither Sd(a+) nor Sd(a-) THGPs reacted with two other GalNAc alpha-anomer specific lectins: Codium fragile subspecies tomentosoides and Artocarpus integrifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B4 and native Sd(a+)THGP-DBA through GalNAc beta--> was confirmed by inhibition assay. These results demonstrate that DBA and VVA-B4 are useful reagents to differentiate between Sd(a+) and Sd(a-) THGP.

摘要

通过定量沉淀试验（QPA）和酶联凝集素吸附试验（ELLSA），研究了人类血型Sd(a+)和Sd(a-) Tamm-Horsfall糖蛋白（THGPs）与四种GalNAc特异性凝集素的结合模式。在通过QPA和ELLSA检测的天然及去唾液酸Sd(a+)和Sd(a-) THGP中，只有天然及去唾液酸Sd(a+)能与双花扁豆（DBA）和绒毛野豌豆-B4（VVA-B4）良好结合，而Sd(a-) THGP与这两种凝集素反应较差。Sd(a+)和Sd(a-) THGPs均不与另外两种GalNAcα-端基异构体特异性凝集素反应：柔弱松节藻绒毛亚种和波罗蜜。此外，通过抑制试验证实了去唾液酸Sd(a+)THGP-VVA-B4和天然Sd(a+)THGP-DBA通过GalNAcβ-的结合。这些结果表明，DBA和VVA-B4是区分Sd(a+)和Sd(a-) THGP的有用试剂。