Scott P M
Food Research Division, Bureau of Chemical Safety, Health Canada, Ottawa, Ontario.
Food Addit Contam. 1995 May-Jun;12(3):395-403. doi: 10.1080/02652039509374321.
Sensitive, specific, accurate and precise methods of analysis are needed for enforcement of mycotoxin regulations, other monitoring programmes, and research studies. Rapid screening tests are useful for control at all stages of food and feed production. There is a wide choice of both quantitative and qualitative methods for the more well known mycotoxins. Those at present covered by method standardization organizations such as AOAC International are aflatoxins (including M1), Alternaria toxins, citrinin, cyclopiazonic acid, ergot alkaloids, fumonisins, ochratoxins, patulin, trichothecenes, and zearalenone. Methodology for mycotoxins is selectively reviewed in this paper with emphasis on the procedures comprising the analytical method--sampling, extraction of naturally contaminated samples, clean-up, detection and determination, and confirmation. Also covered are automation, method comparison, and method assessment.
执行霉菌毒素法规、开展其他监测计划以及进行研究都需要灵敏、特异、准确且精密的分析方法。快速筛选检测对于食品和饲料生产各阶段的控制很有用。对于较为知名的霉菌毒素，有大量定量和定性方法可供选择。目前由诸如美国官方分析化学师协会（AOAC International）等方法标准化组织涵盖的霉菌毒素包括黄曲霉毒素（包括M1）、链格孢菌毒素、桔霉素、环匹阿尼酸、麦角生物碱、伏马菌素、赭曲霉毒素、展青霉素、单端孢霉烯族毒素和玉米赤霉烯酮。本文对霉菌毒素的方法进行了选择性综述，重点关注构成分析方法的程序——采样、天然污染样品的提取、净化、检测与测定以及确证。还涵盖了自动化、方法比较和方法评估。
