Fasano S, Chieffi P, Minucci S, Le Guellec K, Jégou B, Pierantoni R
Dipartimento di Fisiologia Umana e Funzioni Biologiche Integrate F. Bottazzi, Facoltà di Medicina, Il Università di Napoli, Italy.
Mol Cell Endocrinol. 1995 Apr 1;109(2):127-32. doi: 10.1016/0303-7207(95)03495-s.
The objective of the present paper was to do a comparative study to assess somatic versus germ cell localization of c-mos products in the testis. In mouse and amphibian oocytes, c-mos activity is necessary for meiotic maturation. Lack of c-mos expression has been reported in somatic cells of male and female gonads while transcripts have been found in germ cells of testis and ovary. Using a v-mos probe, we report here the detection of a c-mos related transcript (1.7 kb) in the dogfish Scyliorhinus canicula testis. Western blot analysis detects two proteins of 106 and 32 kDa. A specific immunostaining was exclusively localized in the interstitial tissue while the germinal compartment was completely negative. In conclusion, our results indicate for the first time the presence of c-mos products in an elasmobranch species and, moreover, their presence in somatic testicular cells rather than germ cells. Therefore, this finding in an ancient vertebrate indicates that c-mos activity does not have a direct universal role in the regulation of spermatogenesis.
本文的目的是进行一项比较研究，以评估睾丸中c-mos产物的体细胞定位与生殖细胞定位。在小鼠和两栖类卵母细胞中，c-mos活性对于减数分裂成熟是必需的。据报道，在雄性和雌性性腺的体细胞中缺乏c-mos表达，而在睾丸和卵巢的生殖细胞中发现了转录本。使用v-mos探针，我们在此报告在角鲨（Scyliorhinus canicula）睾丸中检测到一种与c-mos相关的转录本（1.7 kb）。蛋白质免疫印迹分析检测到两种分别为106 kDa和32 kDa的蛋白质。特异性免疫染色仅定位于间质组织，而生发区则完全呈阴性。总之，我们的结果首次表明在一种软骨鱼类中存在c-mos产物，而且它们存在于睾丸体细胞而非生殖细胞中。因此，在一种古老脊椎动物中的这一发现表明，c-mos活性在精子发生的调控中并不具有直接的普遍作用。
