Lee W J, Brey P T
Unité d'Ecologie des Systèmes Vectoriels, Institut Pasteur, Paris, France.
Gene. 1995 Aug 19;161(2):199-203. doi: 10.1016/0378-1119(95)00199-g.
We have isolated and characterized a Bombyx mori (Bm) cDNA encoding a lysozyme (Lyz). A 90-bp DNA fragment was amplified by PCR using degenerate oligodeoxyribonucleotide primers derived from the known amino acid (aa) sequence of the Bm Lyz. These PCR fragments were used to screen a fat body cDNA library. A clone containing the complete lys cDNA (1294 bp) was isolated and completely sequenced. The deduced 137-aa sequence showed high homology with other chicken-type Lyz. Bm lys gene expression was constitutive in fat body, cuticular epidermal tissue and at a very low level in hemocytes. This gene expression was up-regulated in fat body, hemocytes and cuticular epidermal tissue following the injection of Gram+ bacteria.
我们已经分离并鉴定了家蚕（Bombyx mori，Bm）中一个编码溶菌酶（Lyz）的cDNA。使用从Bm Lyz已知氨基酸序列推导而来的简并寡脱氧核糖核苷酸引物，通过PCR扩增出一个90 bp的DNA片段。这些PCR片段用于筛选脂肪体cDNA文库。分离出一个包含完整溶菌酶cDNA（1294 bp）的克隆并进行了全序列测定。推导的137个氨基酸序列与其他鸡型溶菌酶具有高度同源性。Bm溶菌酶基因在脂肪体、表皮组织中组成性表达，在血细胞中表达水平极低。在注射革兰氏阳性菌后，该基因在脂肪体、血细胞和表皮组织中的表达上调。
