Abdel-Banat B M, Koga D
Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi University, 753-8515, Yamaguchi, Japan.
Insect Biochem Mol Biol. 2001 Mar 15;31(4-5):497-508. doi: 10.1016/s0965-1748(00)00160-0.
A genomic clone containing the chitinase gene was isolated and characterized from the silkworm, Bombyx mori, using the polymerase chain reaction technique directed by primers designed from the chitinase cDNA of the insect previously cloned [Insect Biochem. Molec. Biol. 28 (1998) 163]. Results from nucleotide sequence analysis of the gene and its PCR amplification suggest that the B. mori genome probably has only one copy of chitinase gene. The gene is organized into 10 coding regions, exons, interrupted by noncoding regions, introns. The motifs encoded and exon organization of the gene are almost identical to the related gene from Manduca sexta. The results suggest that the domain organization of chitinase genes may be conserved among insect cuticular chitinase genes and that they are more complex than their counterparts in plants. The possibilities of intron splicing from the primary transcripts are also discussed.
利用先前克隆的昆虫几丁质酶cDNA设计的引物，通过聚合酶链反应技术，从家蚕中分离并鉴定了一个包含几丁质酶基因的基因组克隆[《昆虫生物化学与分子生物学》28(1998)163]。该基因的核苷酸序列分析及其PCR扩增结果表明，家蚕基因组中可能只有一个几丁质酶基因拷贝。该基因由10个编码区（外显子）组成，被非编码区（内含子）打断。该基因编码的基序和外显子组织与烟草天蛾的相关基因几乎相同。结果表明，几丁质酶基因的结构域组织在昆虫表皮几丁质酶基因中可能是保守的，并且它们比植物中的对应基因更复杂。还讨论了从初级转录本中进行内含子剪接的可能性。
