来自H2-M区域的大于1.5兆碱基酵母人工染色体重叠群中的MHC I类基因组织。

MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region.

作者信息

Jones E P, Xiao H, Schultz R A, Flaherty L, Trachtulec Z, Vincek V, Larin Z, Lehrach H, Lindahl K F

机构信息

Howard Hughes Medical Institute, Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9050, USA.

出版信息

Genomics. 1995 May 1;27(1):40-51. doi: 10.1006/geno.1995.1006.

DOI:10.1006/geno.1995.1006

PMID:7665183

Abstract

Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single- and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A parallel M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8.

摘要

16个酵母人工染色体（YAC）克隆已被定位到位于小鼠第17号染色体主要组织相容性复合体（MHC）远端的H2-M区域。用单拷贝和多拷贝探针分析这些YAC，得到一个至少跨越800 kb的近端重叠群和一个700 kb的远端重叠群。一个针对MHC I类基因保守的第四外显子的探针在近端重叠群中检测到19个限制性片段，包括8个先前已鉴定的H2-M I类基因中的6个。这个重叠群跨越了从M区域到T区域的间隙，并包括T1基因。相比之下，在远端重叠群中仅发现两个I类基因，即M2和M3。这两个都表达的基因可能标志着MHC的末端。在来自着丝粒的克隆DNA中确定了9个I类基因和7个其他标记之间的顺序为T1、Tu32A、（M1-M7-M8）、Tu32B、B30、M6、M4、M5、Mog、Tu42A平行于M2、Leh525、M3、Tu42B，其中M1-M7-M8相对于着丝粒的方向未知。