Patwardhan V G, Jaswaney V L, Pal J K, Modak S P
Department of Zoology, University of Poona, Pune, India.
Indian J Biochem Biophys. 1995 Feb;32(1):21-31.
Antisera prepared against total water-soluble lens proteins of the shark, Scoliodon sorrakowah were reacted with homologous antigen and analysed reaction products by immunoelectrophoresis (IE) and two dimensional crossed antigen-antibody electrophoresis (2D-CE). On IE, shark antigens formed 5 precipitin lines including 1 alpha, 3 beta and 1 gamma crystallins and on 2D-CE 3 alpha, 6 beta and 6 gamma peaks accounting for 8%, 27% and 65% antigen in the respective group were obtained from the total crystallins. Using anti-shark antisera, the immunocrossreactivity of lens proteins from 6 Chondropterygii, 23 teleosts and 16 higher vertebrates was examined by IE. It is found that beta crystallins are the most conserved and crossreact with all vertebrate classes, whereas gamma crystallin crossreactivity is specific to the class Pisces and alpha crystallins are least conserved and their crossreactivity is confined to subclass Chondropterygii. Based on IE patterns, a phylogenetic tree is constructed which demonstrates the intrafamily closeness except in case of adaptive radiation.
用制备的抗皱唇鲨（Scoliodon sorrakowah）全水溶性晶状体蛋白的抗血清与同源抗原反应，并通过免疫电泳（IE）和二维交叉抗原-抗体电泳（2D-CE）分析反应产物。在免疫电泳中，鲨鱼抗原形成5条沉淀线，包括1条α、3条β和1条γ晶状体蛋白；在二维交叉抗原-抗体电泳中，从总晶状体蛋白中获得了3个α峰、6个β峰和6个γ峰，分别占各自组中抗原的8%、27%和65%。使用抗鲨鱼抗血清，通过免疫电泳检测了6种软骨鱼类、23种硬骨鱼类和16种高等脊椎动物晶状体蛋白的免疫交叉反应性。发现β晶状体蛋白最保守，与所有脊椎动物类群都有交叉反应，而γ晶状体蛋白的交叉反应性特定于鱼类，α晶状体蛋白最不保守，其交叉反应性局限于软骨鱼亚纲。根据免疫电泳图谱构建了系统发育树，该树显示了除适应性辐射外的科内亲缘关系。
