Radford K J, Mallesch J, Hersey P
Oncology and Immunology Unit, David Maddison Clinical Sciences Bldg., Newcastle, NSW 2300, Australia.
Int J Cancer. 1995 Sep 4;62(5):631-5. doi: 10.1002/ijc.2910620523.
CD63 has been identified in human melanoma cells by a number of different monoclonal antibodies (MAbs). Studies with MAbs have shown that expression is most marked in naevi and early forms of cutaneous melanoma and reduced in vertical growth phase and metastatic lesions. To investigate further the role of CD63 in progression of melanoma, genomic CD63 was transfected into a CD63-negative human melanoma cell line using an episomal vector. The stable transfected melanoma cells had similar growth rates to control transfected melanoma cells in vitro but much lower growth rates when injected intradermally into athymic nude mice. The CD63-transfected cells also had a reduced number of metastases in the peritoneal cavity and subcutaneous sites when injected intravenously. MAb against CD63 did not influence the growth of CD63-transfected melanoma cells in vitro. Our results confirm previous studies using H-ras-transformed NIH3T3 fibroblasts and suggest that CD63 may have a role as a tumor suppressor gene in human melanoma that acts to limit invasion and progression of melanoma.
多种不同的单克隆抗体（MAb）已在人类黑色素瘤细胞中鉴定出CD63。对单克隆抗体的研究表明，其表达在痣和皮肤黑色素瘤的早期形式中最为明显，而在垂直生长期和转移病灶中则减少。为了进一步研究CD63在黑色素瘤进展中的作用，使用游离型载体将基因组CD63转染到CD63阴性的人类黑色素瘤细胞系中。稳定转染的黑色素瘤细胞在体外与对照转染的黑色素瘤细胞具有相似的生长速率，但皮内注射到无胸腺裸鼠体内时生长速率要低得多。静脉注射时，CD63转染的细胞在腹腔和皮下部位的转移数量也减少。抗CD63的单克隆抗体在体外不影响CD63转染的黑色素瘤细胞的生长。我们的结果证实了先前使用H-ras转化的NIH3T3成纤维细胞的研究，并表明CD63可能作为人类黑色素瘤中的一种肿瘤抑制基因，起到限制黑色素瘤侵袭和进展的作用。
