Lai Xiaojing, Gu Qing, Zhou Xia, Feng Wei, Lin Xiao, He Yan, Cao Jinming, Liu Pengfei, Zhang Huojun, Zheng Xiao
Department of Radiation Oncology, Zhejiang Key Laboratory of Diagnosis and Treatment Technology on Thoracic Oncology (Lung and Esophagus), Zhejiang Cancer Hospital (Zhejiang Cancer Research Institute), Hangzhou, Zhejiang 310022, P.R. China.
School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Soochow, Suzhou 215123, P.R. China.
Oncol Lett. 2017 Jun;13(6):4245-4251. doi: 10.3892/ol.2017.6023. Epub 2017 Apr 11.
The tetraspanin CD63 has been described to have critical roles in multiple biological processes, including tumorigenesis and metastasis in several types of cancer. However, its role in esophageal carcinoma (EC) has not been reported. In the current study, immunohistochemistry was used to investigate CD63 expression in 106 esophageal cancer samples, 49 adjacent esophagus tissues and 17 normal esophagus mucosa tissues. The results revealed that the overexpression of CD63 was observed in esophageal cancer samples and negatively correlated with tumor stage and lymph node metastasis. To further evaluate the role of CD63 in esophageal carcinoma, the invasiveness of EC cells was analyzed using matrigel invasion assays and wound healing assays . Furthermore, it was found that CD63 knockdown increased the invasiveness of TE-1 cells through the upregulation of matrix metalloproteinase (MMP) expression via promoting epithelial-mesenchymal transition. The current data therefore suggested that low levels of CD63 expression may be involved in the tumor progression of esophageal carcinoma.
四跨膜蛋白CD63已被描述在多种生物学过程中发挥关键作用，包括在几种类型癌症的肿瘤发生和转移过程中。然而，其在食管癌（EC）中的作用尚未见报道。在本研究中，采用免疫组织化学方法检测了106例食管癌样本、49例癌旁食管组织和17例正常食管黏膜组织中CD63的表达情况。结果显示，食管癌样本中观察到CD63过表达，且与肿瘤分期和淋巴结转移呈负相关。为进一步评估CD63在食管癌中的作用，使用基质胶侵袭实验和伤口愈合实验分析了EC细胞的侵袭能力。此外，发现敲低CD63可通过促进上皮-间质转化上调基质金属蛋白酶（MMP）的表达，从而增加TE-1细胞的侵袭能力。因此，目前的数据表明，低水平的CD63表达可能参与食管癌的肿瘤进展。
