Lam T J, Pengov A, Schukken Y H, Smit J A, Brand A
Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, Utrecht, The Netherlands.
J Appl Bacteriol. 1995 Jul;79(1):69-72. doi: 10.1111/j.1365-2672.1995.tb03125.x.
Haemolysin production, the slide coagulase test and the tube coagulase test were assessed for their capability to differentiate Staphylococcus aureus among other Micrococcaceae in 199 isolates from udders of cows in herds with a low bulk milk somatic cell count. The API-Staph test was used as a reference. Haemolysin production was less effective in identifying Staph. aureus among Micrococcaceae than a combination of other tests. Differences were found in the predictive values of results from diagnostic protocols in which the slide coagulase test was performed on all Micrococcaceae, or on beta-haemolysin-negative Micrococcaceae only. Diagnostic protocols in which haemolysin production was combined with the results of the other tests resulted in excellent diagnostic performance and a reduction in diagnostic procedures. Recommendations for routine Staph. aureus identification in bovine mastitis bacteriology are given.
对199株来自低批量牛奶体细胞计数牛群乳房的菌株进行溶血素产生、玻片凝固酶试验和试管凝固酶试验，以评估它们在区分金黄色葡萄球菌和其他微球菌科细菌方面的能力。使用API - Staph试验作为参考。在微球菌科细菌中，溶血素产生在鉴定金黄色葡萄球菌方面不如其他试验组合有效。在对所有微球菌科细菌或仅对β - 溶血素阴性微球菌科细菌进行玻片凝固酶试验的诊断方案中，结果的预测值存在差异。将溶血素产生与其他试验结果相结合的诊断方案具有出色的诊断性能，并减少了诊断程序。给出了牛乳腺炎细菌学中常规鉴定金黄色葡萄球菌的建议。
