Wagar E A, Giese M J, Yasin B, Pang M
Department of Pathology, UCLA School of Medicine 90095, USA.
J Bacteriol. 1995 Sep;177(17):5179-85. doi: 10.1128/jb.177.17.5179-5185.1995.
Aminoacyl-tRNA synthetases specifically charge tRNAs with their cognate amino acids. A prototype for the most complex aminoacyl-tRNA synthetases is the four-subunit glycyl-tRNA synthetase from Escherichia coli, encoded by two open reading frames. We examined the glycyl-tRNA synthetase gene from Chlamydia trachomatis, a genetically isolated bacterium, and identified only a single open reading frame for the chlamydial homolog (glyQS). This is the first report of a prokaryotic glycyl-tRNA synthetase encoded by a single gene.
氨酰-tRNA合成酶特异性地将其对应的氨基酸加载到tRNA上。最复杂的氨酰-tRNA合成酶的一个原型是来自大肠杆菌的由两个开放阅读框编码的四亚基甘氨酰-tRNA合成酶。我们研究了沙眼衣原体（一种基因上孤立的细菌）的甘氨酰-tRNA合成酶基因，并确定衣原体同源物（glyQS）只有一个开放阅读框。这是关于由单个基因编码的原核甘氨酰-tRNA合成酶的首次报道。
