ntrBC突变对深红红螺菌中固氮酶活性翻译后调控的影响。
Effect of an ntrBC mutation on the posttranslational regulation of nitrogenase activity in Rhodospirillum rubrum.
作者信息
Zhang Y, Cummings A D, Burris R H, Ludden P W, Roberts G P
机构信息
Department of Biochemistry, University of Wisconsin-Madison 53706, USA.
出版信息
J Bacteriol. 1995 Sep;177(18):5322-6. doi: 10.1128/jb.177.18.5322-5326.1995.
Abstract
Homologs of ntrB and ntrC genes from Rhodospirillum rubrum were cloned and sequenced. A mutant lacking ntrBC was constructed, and this mutant has normal nitrogenase activity under nif-derepressing conditions, indicating that ntrBC are not necessary for the expression of the nif genes in R. rubrum. However, the post-translational regulation of nitrogenase activity by ADP-ribosylation in response to NH4+ was partially abolished in this mutant. More surprisingly, the regulation of nitrogenase activity in response to darkness was also affected, suggesting a physiological link between the ntr system and energy signal transduction in R. rubrum. The expression of glutamine synthetase, as well as its posttranslational regulation, was also altered in this ntrBC mutant.
摘要
对来自深红红螺菌的ntrB和ntrC基因的同源物进行了克隆和测序。构建了一个缺乏ntrBC的突变体，该突变体在固氮酶去阻遏条件下具有正常的固氮酶活性，这表明ntrBC对于深红红螺菌中nif基因的表达不是必需的。然而，该突变体中由ADP-核糖基化对NH4+响应的固氮酶活性的翻译后调控被部分消除。更令人惊讶的是，对黑暗响应的固氮酶活性调控也受到影响，这表明深红红螺菌中ntr系统与能量信号转导之间存在生理联系。该ntrBC突变体中谷氨酰胺合成酶的表达及其翻译后调控也发生了改变。
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