Cheng J, Johansson M, Nordlund S
Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden.
J Bacteriol. 1999 Oct;181(20):6530-4. doi: 10.1128/JB.181.20.6530-6534.1999.
We have studied the transcription of the glnB and glnA genes in Rhodospirillum rubrum with firefly luciferase as a reporter enzyme. Under NH(4)(+) and N(2) conditions, glnBA was cotranscribed from a weak and a strong promoter. In nitrogen-fixing cultures, activity of the latter was highly enhanced by NtrC, but transcription from both promoters occurred under both conditions. There is no promoter controlling transcription of glnA alone, supporting our proposal that the glnA mRNA is produced by processing.
我们以萤火虫荧光素酶作为报告酶,研究了深红红螺菌中glnB和glnA基因的转录情况。在NH₄⁺和N₂条件下,glnBA从一个弱启动子和一个强启动子共同转录。在固氮培养物中,后者的活性被NtrC高度增强,但在两种条件下两个启动子都发生转录。不存在单独控制glnA转录的启动子,这支持了我们关于glnA mRNA是通过加工产生的提议。