产酸克雷伯菌8017的araE基因的克隆、测序及表达，该基因编码阿拉伯糖-H⁺同向转运活性。
Cloning, sequencing, and expression of the araE gene of Klebsiella oxytoca 8017, which encodes arabinose-H+ symport activity.
作者信息
Shatwell K P, Charalambous B M, McDonald T P, Henderson P J
机构信息
Department of Biochemistry, University of Cambridge, United Kingdom.
出版信息
J Bacteriol. 1995 Sep;177(18):5379-80. doi: 10.1128/jb.177.18.5379-5380.1995.
Abstract
Southern analysis of the genomic DNA from species of the family Enterobacteriaceae, using a probe derived from the Escherichia coli araE gene, which encodes an arabinose-H+ symporter, detected araE in Salmonella, Citrobacter, Klebsiella, and Enterobacter spp. The Klebsiella oxytoca araE gene was cloned, sequenced, and expressed to compare its properties with those of araE from E. coli.
摘要
利用源自大肠杆菌araE基因（编码阿拉伯糖-H⁺同向转运体）的探针，对肠杆菌科细菌的基因组DNA进行Southern印迹分析，结果在沙门氏菌、柠檬酸杆菌、克雷伯氏菌和肠杆菌属中检测到了araE。对产酸克雷伯氏菌的araE基因进行了克隆、测序和表达，以将其特性与大肠杆菌的araE特性进行比较。
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