荧光假单胞菌CHA0中管家σ因子的扩增增强了抗生素的产生并提高了生物防治能力。
Amplification of the housekeeping sigma factor in Pseudomonas fluorescens CHA0 enhances antibiotic production and improves biocontrol abilities.
作者信息
Schnider U, Keel C, Blumer C, Troxler J, Défago G, Haas D
机构信息
Laboratoire de Biologie Microbienne, Université de Lausanne, Switzerland.
出版信息
J Bacteriol. 1995 Sep;177(18):5387-92. doi: 10.1128/jb.177.18.5387-5392.1995.
Abstract
Pseudomonas fluorescens CHA0 produces a variety of secondary metabolites, in particular the antibiotics pyoluteorin and 2,4-diacetylphloroglucinol, and protects various plants from diseases caused by soilborne pathogenic fungi. The rpoD gene encoding the housekeeping sigma factor sigma 70 of P. fluorescens was sequenced. The deduced RpoD protein showed 83% identity with RpoD of Pseudomonas aeruginosa and 67% identity with RpoD of Escherichia coli. Attempts to inactivate the single chromosomal rpoD gene of strain CHA0 were unsuccessful, indicating an essential role of this gene. When rpoD was carried by an IncP vector in strain CHA0, the production of both antibiotics was increased severalfold and, in parallel, protection of cucumber against disease caused by Pythium ultimum was improved, in comparison with strain CHA0.
摘要
荧光假单胞菌CHA0能产生多种次生代谢产物，特别是抗生素绿脓菌素和2,4 - 二乙酰基间苯三酚，并能保护多种植物免受土传致病真菌引起的病害。对编码荧光假单胞菌管家σ因子σ70的rpoD基因进行了测序。推导的RpoD蛋白与铜绿假单胞菌的RpoD有83%的同一性，与大肠杆菌的RpoD有67%的同一性。使CHA0菌株的单个染色体rpoD基因失活的尝试未成功，表明该基因具有重要作用。当rpoD由IncP载体携带在CHA0菌株中时，与CHA0菌株相比，两种抗生素的产量均增加了数倍，同时，对黄瓜抵御终极腐霉引起的病害的保护作用也得到了改善。
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EMBO J. 1989 Feb;8(2):351-8. doi: 10.1002/j.1460-2075.1989.tb03384.x.
2
Cloning of Genes Involved in the Synthesis of Pyrrolnitrin from Pseudomonas fluorescens and Role of Pyrrolnitrin Synthesis in Biological Control of Plant Disease.从荧光假单胞菌中克隆涉及吡咯并硝酮合成的基因及其在植物病害生物防治中的作用。
Appl Environ Microbiol. 1994 Jan;60(1):78-85. doi: 10.1128/aem.60.1.78-85.1994.
3
Heterogeneity of the principal sigma factor in Escherichia coli: the rpoS gene product, sigma 38, is a second principal sigma factor of RNA polymerase in stationary-phase Escherichia coli.大肠杆菌中主要σ因子的异质性：rpoS基因产物σ38是静止期大肠杆菌中RNA聚合酶的第二个主要σ因子。
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3511-5. doi: 10.1073/pnas.90.8.3511.
4
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J Bacteriol. 1994 Jul;176(13):3966-74. doi: 10.1128/jb.176.13.3966-3974.1994.
5
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Annu Rev Microbiol. 1994;48:53-80. doi: 10.1146/annurev.mi.48.100194.000413.
6
The trials and tribulations of growth arrest.生长停滞的艰难困苦。
Trends Microbiol. 1995 Apr;3(4):131-6. doi: 10.1016/s0966-842x(00)88901-5.
7
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Mol Microbiol. 1994 Sep;13(6):1071-7. doi: 10.1111/j.1365-2958.1994.tb00498.x.
8
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9
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