用比色法测定液体培养基中鸟分枝杆菌-胞内分枝杆菌复合群的最低抑菌浓度。
Determination of MICs for Mycobacterium avium-M. intracellulare complex in liquid medium by a colorimetric method.
作者信息
Gomez-Flores R, Gupta S, Tamez-Guerra R, Mehta R T
机构信息
University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
出版信息
J Clin Microbiol. 1995 Jul;33(7):1842-6. doi: 10.1128/jcm.33.7.1842-1846.1995.
Abstract
We investigated the potential of a rapid colorimetric microassay based on the reduction of dimethylthiazol-diphenyltetrazolium bromide (MTT) for determining the growth of Mycobacterium avium-M. intracellulare complex (MAC) and MICs of clofazimine, resorcinomycin A, and the quinolone PD 127391 against MAC. The reduction of MTT was directly proportional to the number of viable bacteria. A comparison of the MTT reduction test with the [3H]glycerol uptake assay showed the former to possess higher analytical sensitivity for detecting MAC growth in microtiter plates. The MIT reduction test avoids the use of radioisotopes and costly material and equipment; it is reliable, reproducible, and convenient for rapid routine susceptibility testing of MAC.
摘要
我们研究了基于溴化二甲噻唑二苯基四氮唑（MTT）还原反应的快速比色微量测定法在测定鸟分枝杆菌-胞内分枝杆菌复合体（MAC）生长以及氯法齐明、间苯二酚霉素A和喹诺酮PD 127391对MAC的最低抑菌浓度（MIC）方面的潜力。MTT的还原与活菌数量成正比。将MTT还原试验与[³H]甘油摄取试验进行比较，结果表明前者在检测微量滴定板中MAC生长方面具有更高的分析灵敏度。MTT还原试验避免了使用放射性同位素以及昂贵的材料和设备；它可靠、可重复，并且便于对MAC进行快速常规药敏试验。
相似文献
1
Determination of MICs for Mycobacterium avium-M. intracellulare complex in liquid medium by a colorimetric method.用比色法测定液体培养基中鸟分枝杆菌-胞内分枝杆菌复合群的最低抑菌浓度。
J Clin Microbiol. 1995 Jul;33(7):1842-6. doi: 10.1128/jcm.33.7.1842-1846.1995.
2
Enhanced intramacrophage activity of resorcinomycin A against Mycobacterium avium-Mycobacterium intracellulare complex after liposome encapsulation.脂质体包封后，间苯二酚霉素A对鸟分枝杆菌-胞内分枝杆菌复合群的巨噬细胞内活性增强。
Antimicrob Agents Chemother. 1996 Nov;40(11):2545-9. doi: 10.1128/AAC.40.11.2545.
3
In vitro activity of aminoglycosides, clofazimine, d-cycloserine and dapsone against 83 Mycobacterium avium complex clinical isolates.氨基糖苷类、氯法齐明、环丝氨酸和氨苯砜对 83 株鸟分枝杆菌复合体临床分离株的体外活性。
J Microbiol Immunol Infect. 2018 Oct;51(5):636-643. doi: 10.1016/j.jmii.2017.05.001. Epub 2017 Jun 28.
4
In vitro activities of free and liposomal drugs against Mycobacterium avium-M. intracellulare complex and M. tuberculosis.游离药物和脂质体药物对鸟分枝杆菌-胞内分枝杆菌复合群及结核分枝杆菌的体外活性。
Antimicrob Agents Chemother. 1993 Dec;37(12):2584-7. doi: 10.1128/AAC.37.12.2584.
5
Differences in drug susceptibility pattern between Mycobacterium avium and Mycobacterium intracellulare isolated in respiratory specimens.呼吸道标本中分离出的鸟分枝杆菌和胞内分枝杆菌之间药物敏感性模式的差异。
J Infect Chemother. 2018 Apr;24(4):315-318. doi: 10.1016/j.jiac.2017.10.022. Epub 2017 Dec 7.
6
French multicenter study involving eight test sites for radiometric determination of activities of 10 antimicrobial agents against Mycobacterium avium complex.一项法国多中心研究，涉及八个试验点，用于放射性测定10种抗菌药物对鸟分枝杆菌复合群的活性。
Antimicrob Agents Chemother. 1995 Mar;39(3):638-44. doi: 10.1128/AAC.39.3.638.
7
Susceptibility of Mycobacterium avium and Mycobacterium intracellulare to various antibacterial drugs.鸟分枝杆菌和胞内分枝杆菌对各种抗菌药物的敏感性。
Microbiol Immunol. 1989;33(6):509-14. doi: 10.1111/j.1348-0421.1989.tb02000.x.
8
Liposome encapsulation of clofazimine reduces toxicity in vitro and in vivo and improves therapeutic efficacy in the beige mouse model of disseminated Mycobacterium avium-M. intracellulare complex infection.氯法齐明的脂质体包封可降低体外和体内毒性，并提高播散性鸟分枝杆菌-胞内分枝杆菌复合感染米色小鼠模型的治疗效果。
Antimicrob Agents Chemother. 1996 Aug;40(8):1893-902. doi: 10.1128/AAC.40.8.1893.
9
[In vitro susceptibilities of Mycobacterium avium and Mycobacterium intracellulare to various drugs].鸟分枝杆菌和胞内分枝杆菌对多种药物的体外敏感性
Kekkaku. 1991 Jul;66(7):489-92.
10
N-1-tert-butyl-substituted quinolones: in vitro anti-Mycobacterium avium activities and structure-activity relationship studies.N-1-叔丁基取代喹诺酮类：体外抗鸟分枝杆菌活性及构效关系研究
Antimicrob Agents Chemother. 1996 Nov;40(11):2637-43. doi: 10.1128/AAC.40.11.2637.
引用本文的文献
1
Exploring the antimicrobial potential of lactobacilli against early-stage and mature biofilms of and .探索乳酸杆菌对[具体细菌名称1]和[具体细菌名称2]早期和成熟生物膜的抗菌潜力。
Front Chem. 2025 Mar 21;13:1425666. doi: 10.3389/fchem.2025.1425666. eCollection 2025.
2
Exploring the Antimycobacterial Potential of Podocarpusflavone A from : In Vitro and In Vivo Insights.探索来自罗汉松黄酮A的抗分枝杆菌潜力：体外和体内研究见解
Pharmaceuticals (Basel). 2024 Nov 21;17(12):1560. doi: 10.3390/ph17121560.
3
UHPLC-HRMS/MS Chemical Fingerprinting of the Bioactive Partition from Cultivated L.超高效液相色谱-高分辨质谱联用对栽培紫苏生物活性部位的化学指纹图谱分析
Molecules. 2024 Apr 9;29(8):1690. doi: 10.3390/molecules29081690.
4
Phosphopantetheinyl transferase binding and inhibition by amidino-urea and hydroxypyrimidinethione compounds.磷酸泛酰巯基乙胺转移酶结合和抑制的酰胺基脲和羟嘧啶硫酮化合物。
Sci Rep. 2021 Sep 10;11(1):18042. doi: 10.1038/s41598-021-97197-4.
5
The Contribution of Efflux Pumps in Complex Resistance to Clarithromycin.外排泵在对克拉霉素的复杂耐药性中的作用
Antibiotics (Basel). 2019 Sep 18;8(3):153. doi: 10.3390/antibiotics8030153.
6
Impairment of Cronobacter sakazakii and Listeria monocytogenes biofilms by cell-free preparations of lactobacilli of goat milk origin.干酪乳杆菌细胞游离提取物对阪崎克罗诺杆菌和单核细胞增生李斯特菌生物膜的抑制作用。
Folia Microbiol (Praha). 2020 Feb;65(1):185-196. doi: 10.1007/s12223-019-00721-3. Epub 2019 Jun 19.
7
N-acetyl-cysteine exhibits potent anti-mycobacterial activity in addition to its known anti-oxidative functions.N-乙酰半胱氨酸除了具有已知的抗氧化功能外，还表现出强大的抗分枝杆菌活性。
BMC Microbiol. 2016 Oct 28;16(1):251. doi: 10.1186/s12866-016-0872-7.
8
Nitric Oxide Production Inhibition and Anti-Mycobacterial Activity of Extracts and Halogenated Sesquiterpenes from the Brazilian Red Alga Laurencia Dendroidea J. Agardh.巴西红藻劳伦氏树状藻提取物和卤化倍半萜的一氧化氮生成抑制及抗分枝杆菌活性
Pharmacogn Mag. 2015 Oct;11(Suppl 4):S611-8. doi: 10.4103/0973-1296.172972.
9
Enhancement of antibiotic activity by efflux inhibitors against multidrug resistant Mycobacterium tuberculosis clinical isolates from Brazil.外排抑制剂对巴西多药耐药结核分枝杆菌临床分离株抗生素活性的增强作用。
Front Microbiol. 2015 Apr 28;6:330. doi: 10.3389/fmicb.2015.00330. eCollection 2015.
10
Antimycobacterial and nitric oxide production inhibitory activities of Ocotea notata from Brazilian restinga.巴西滨海沙质地植被中诺塔桂叶樟的抗分枝杆菌及一氧化氮生成抑制活性
ScientificWorldJournal. 2015;2015:947248. doi: 10.1155/2015/947248. Epub 2015 Feb 19.
本文引用的文献
1
In vitro activities of free and liposomal drugs against Mycobacterium avium-M. intracellulare complex and M. tuberculosis.游离药物和脂质体药物对鸟分枝杆菌-胞内分枝杆菌复合群及结核分枝杆菌的体外活性。
Antimicrob Agents Chemother. 1993 Dec;37(12):2584-7. doi: 10.1128/AAC.37.12.2584.
2
Factors affecting the selection and use of tetrazolium salts as cytochemical indicators of microbial viability and activity.影响四氮唑盐作为微生物活力和活性细胞化学指示剂选择与使用的因素。
J Appl Bacteriol. 1993 Apr;74(4):433-43. doi: 10.1111/j.1365-2672.1993.tb05151.x.
3
Determination of ansamycin MICs for Mycobacterium avium complex in liquid medium by radiometric and conventional methods.采用放射测量法和传统方法测定液体培养基中鸟分枝杆菌复合群的安莎霉素最低抑菌浓度。
Antimicrob Agents Chemother. 1985 Oct;28(4):570-5. doi: 10.1128/AAC.28.4.570.
4
Comparative in vitro activities of MDL 473, rifampin, and ansamycin against Mycobacterium intracellulare.MDL 473、利福平及安莎霉素对胞内分枝杆菌的体外比较活性
Antimicrob Agents Chemother. 1985 Sep;28(3):440-1. doi: 10.1128/AAC.28.3.440.
5
A one-plate assay for macrophage bactericidal activity.一种用于巨噬细胞杀菌活性的单孔试验。
J Immunol Methods. 1985 Sep 3;82(1):131-40. doi: 10.1016/0022-1759(85)90232-7.
6
Mycobacterium avium complex infection in patients with the acquired immunodeficiency syndrome. A clinicopathologic study.
Chest. 1988 May;93(5):926-32. doi: 10.1378/chest.93.5.926.
7
Qualitative and quantitative drug-susceptibility tests in mycobacteriology.分枝杆菌学中的定性和定量药敏试验。
Am Rev Respir Dis. 1988 May;137(5):1217-22. doi: 10.1164/ajrccm/137.5.1217.
8
Ethambutol MICs and MBCs for Mycobacterium avium complex and Mycobacterium tuberculosis.鸟分枝杆菌复合群和结核分枝杆菌的乙胺丁醇最低抑菌浓度（MICs）和最低杀菌浓度（MBCs）
Antimicrob Agents Chemother. 1986 Dec;30(6):927-32. doi: 10.1128/AAC.30.6.927.
9
Effect of combined therapy with ansamycin, clofazimine, ethambutol, and isoniazid for Mycobacterium avium infection in patients with AIDS.安莎霉素、氯法齐明、乙胺丁醇和异烟肼联合治疗对艾滋病患者鸟分枝杆菌感染的疗效。
J Infect Dis. 1989 Apr;159(4):784-7. doi: 10.1093/infdis/159.4.784.
10
Isolation and characterization of new antibiotics resorcinomycins A and B.
J Antibiot (Tokyo). 1989 Jan;42(1):1-6. doi: 10.7164/antibiotics.42.1.
Zotero 插件