用比色法测定液体培养基中鸟分枝杆菌-胞内分枝杆菌复合群的最低抑菌浓度。
Determination of MICs for Mycobacterium avium-M. intracellulare complex in liquid medium by a colorimetric method.
作者信息
Gomez-Flores R, Gupta S, Tamez-Guerra R, Mehta R T
机构信息
University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
出版信息
J Clin Microbiol. 1995 Jul;33(7):1842-6. doi: 10.1128/jcm.33.7.1842-1846.1995.
Abstract
We investigated the potential of a rapid colorimetric microassay based on the reduction of dimethylthiazol-diphenyltetrazolium bromide (MTT) for determining the growth of Mycobacterium avium-M. intracellulare complex (MAC) and MICs of clofazimine, resorcinomycin A, and the quinolone PD 127391 against MAC. The reduction of MTT was directly proportional to the number of viable bacteria. A comparison of the MTT reduction test with the [3H]glycerol uptake assay showed the former to possess higher analytical sensitivity for detecting MAC growth in microtiter plates. The MIT reduction test avoids the use of radioisotopes and costly material and equipment; it is reliable, reproducible, and convenient for rapid routine susceptibility testing of MAC.
摘要
我们研究了基于溴化二甲噻唑二苯基四氮唑（MTT）还原反应的快速比色微量测定法在测定鸟分枝杆菌-胞内分枝杆菌复合体（MAC）生长以及氯法齐明、间苯二酚霉素A和喹诺酮PD 127391对MAC的最低抑菌浓度（MIC）方面的潜力。MTT的还原与活菌数量成正比。将MTT还原试验与[³H]甘油摄取试验进行比较，结果表明前者在检测微量滴定板中MAC生长方面具有更高的分析灵敏度。MTT还原试验避免了使用放射性同位素以及昂贵的材料和设备；它可靠、可重复，并且便于对MAC进行快速常规药敏试验。
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本文引用的文献
1
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Antimicrob Agents Chemother. 1993 Dec;37(12):2584-7. doi: 10.1128/AAC.37.12.2584.
2
Factors affecting the selection and use of tetrazolium salts as cytochemical indicators of microbial viability and activity.影响四氮唑盐作为微生物活力和活性细胞化学指示剂选择与使用的因素。
J Appl Bacteriol. 1993 Apr;74(4):433-43. doi: 10.1111/j.1365-2672.1993.tb05151.x.
3
Determination of ansamycin MICs for Mycobacterium avium complex in liquid medium by radiometric and conventional methods.采用放射测量法和传统方法测定液体培养基中鸟分枝杆菌复合群的安莎霉素最低抑菌浓度。
Antimicrob Agents Chemother. 1985 Oct;28(4):570-5. doi: 10.1128/AAC.28.4.570.
4
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Antimicrob Agents Chemother. 1985 Sep;28(3):440-1. doi: 10.1128/AAC.28.3.440.
5
A one-plate assay for macrophage bactericidal activity.一种用于巨噬细胞杀菌活性的单孔试验。
J Immunol Methods. 1985 Sep 3;82(1):131-40. doi: 10.1016/0022-1759(85)90232-7.
6
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Chest. 1988 May;93(5):926-32. doi: 10.1378/chest.93.5.926.
7
Qualitative and quantitative drug-susceptibility tests in mycobacteriology.分枝杆菌学中的定性和定量药敏试验。
Am Rev Respir Dis. 1988 May;137(5):1217-22. doi: 10.1164/ajrccm/137.5.1217.
8
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9
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