Persaud C A, Eykyn S J
Microbiology Department, St Thomas' Hospital, London.
J Clin Pathol. 1995 Jun;48(6):568-70. doi: 10.1136/jcp.48.6.568.
(1) To evaluate the performance of in-house and pre-poured commercially available enteric agar by challenge with a large number of positive clinical specimens. (2) To set the standard (critical independent evaluation) which new products should reach. (3) To publish this information, so that others can make informed decisions about enteric media.
Thirteen media of anonymous source were challenged with "known" positive stool samples.
In-house desoxycholate citrate agar performed best for overall pathogen isolation rates, for shigella isolation rates, and for most pathogens available on primary culture.
Desoxycholate citrate agar made by our own laboratory yielded the most pathogens and proved the most effective.
(1)通过大量阳性临床标本的挑战,评估自制和预灌制的市售肠溶琼脂的性能。(2)设定新产品应达到的标准(关键独立评估)。(3)公布此信息,以便其他人能够对肠道培养基做出明智的决策。
用“已知”阳性粪便样本对13种来源不明的培养基进行挑战。
自制的脱氧胆酸盐柠檬酸盐琼脂在总体病原体分离率、志贺氏菌分离率以及原代培养中可用的大多数病原体方面表现最佳。
我们自己实验室制备的脱氧胆酸盐柠檬酸盐琼脂产生的病原体最多,证明是最有效的。