Traktman P, Caligiuri A, Jesty S A, Liu K, Sankar U
Department of Cell Biology, Cornell University Medical College, New York, NY 10021, USA.
J Virol. 1995 Oct;69(10):6581-7. doi: 10.1128/JVI.69.10.6581-6587.1995.
Vaccinia virus encodes two protein kinases; the B1 kinase is expressed early and appears to play a role during DNA replication, whereas the F10 kinase is expressed late and is encapsidated in virions. Here we report that the F10 kinase gene is the locus affected in a complementation group of temperature-sensitive mutants composed of ts15, ts28, ts54, and ts61. Although these mutants have a biochemically normal phenotype at the nonpermissive temperature, directing the full program of viral gene expression, they fail to form mature virions. Electron microscopic analysis indicates that morphogenesis undergoes arrest at a very early stage, prior to the formation of membrane crescents or immature virions. An essential role for the F10 protein kinase in orchestrating the onset of virion assembly is implied.
痘苗病毒编码两种蛋白激酶；B1激酶早期表达，似乎在DNA复制过程中发挥作用，而F10激酶晚期表达，并被包裹在病毒粒子中。在此我们报告，F10激酶基因是由ts15、ts28、ts54和ts61组成的温度敏感突变体互补组中受影响的基因座。尽管这些突变体在非允许温度下具有生化正常的表型，能指导病毒基因表达的完整程序，但它们无法形成成熟的病毒粒子。电子显微镜分析表明，形态发生在非常早期阶段就停滞了，早于膜新月体或未成熟病毒粒子的形成。这暗示了F10蛋白激酶在协调病毒粒子组装起始过程中起关键作用。
