Vasil'eva S G, Vasil'ev I M
Ontogenez. 1995 May-Jun;26(3):206-12.
In order to study the effect of the stage of development and culturing conditions on the preparation of ES-like cells, we performed experiments on long-term culturing of cow and mouse embryos. We showed that success in isolation of ES-like cells significantly depends on the stage of development. All ES-like cells of cows and mice maintained in culture over three or more transfers were obtained from the embryos at the stage of the released embryo or the late blastocyst, respectively. We found that isolation of ES-like cells of mice was successful only when alpha-MEM medium was used without the addition of BRL-conditioned medium.
为了研究发育阶段和培养条件对类胚胎干细胞制备的影响，我们对牛和小鼠胚胎进行了长期培养实验。我们发现，类胚胎干细胞的分离成功与否很大程度上取决于发育阶段。所有在培养中经过三次或更多次传代的牛和小鼠类胚胎干细胞，分别是从释放胚阶段的胚胎和晚期囊胚中获得的。我们还发现，只有在使用不含BRL条件培养基的α-MEM培养基时，小鼠类胚胎干细胞的分离才能成功。
