de Haan A, Renegar K B, Small P A, Wilschut J
Department of Physiological Chemistry, Groningen Institute for Drug Studies (GIDS), University of Groningen, The Netherlands.
Vaccine. 1995 May;13(7):613-6. doi: 10.1016/0264-410x(94)00062-r.
This study demonstrates that liposomes administered to the lower respiratory tract of mice have the capacity to stimulate secretory IgA (s-IgA) antibody production in the female urogenital system. Total respiratory tract immunization of mice with influenza virus subunit antigen simply mixed with negatively charged liposomes induced antigen-specific s-IgA in vaginal secretions, in addition to systemic IgG and s-IgA in the respiratory tract. Immunization of the upper respiratory tract alone or oral immunization were ineffective. These observations demonstrate that, upon stimulation with liposomes, the lymphoid tissue associated with the lung can act as an inductive site for migration of IgA-committed B cells to distant mucosal tissues, including the female urogenital tract. It is concluded that liposomes administered to the lower respiratory tract provide a promising adjuvant system for stimulation of both systemic and mucosal antibody responses against coadministered antigen, including production of s-IgA at distant mucosal sites.
本研究表明，给予小鼠下呼吸道的脂质体有能力刺激雌性泌尿生殖系统分泌性IgA（s-IgA）抗体的产生。用简单混合了带负电荷脂质体的流感病毒亚单位抗原对小鼠进行全呼吸道免疫，除了在呼吸道诱导产生全身性IgG和s-IgA外，还在阴道分泌物中诱导产生了抗原特异性s-IgA。单独对上呼吸道进行免疫或口服免疫均无效。这些观察结果表明，在用脂质体刺激后，与肺相关的淋巴组织可作为IgA定向B细胞迁移至包括雌性泌尿生殖道在内的远处粘膜组织的诱导部位。得出的结论是，给予下呼吸道的脂质体为刺激针对共同给予抗原的全身性和粘膜抗体反应，包括在远处粘膜部位产生s-IgA，提供了一个有前景的佐剂系统。
