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酿酒酵母七号染色体左臂上一个7941 bp片段的DNA序列包含四个开放阅读框,其中包括pop2突变的多拷贝抑制基因和一个假定的丝氨酸/苏氨酸蛋白激酶基因。

The DNA sequence of a 7941 bp fragment of the left arm of chromosome VII of Saccharomyces cerevisiae contains four open reading frames including the multicopy suppressor gene of the pop2 mutation and a putative serine/threonine protein kinase gene.

作者信息

Coglievina M, Bertani I, Klima R, Zaccaria P, Bruschi C V

机构信息

Department of Microbiology, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.

出版信息

Yeast. 1995 Jun 30;11(8):767-74. doi: 10.1002/yea.320110808.

Abstract

We report the sequence of a 7941 bp DNA fragment from the left arm of chromosome VII of Saccharomyces cerevisiae which contains four open reading frames (ORFs) of greater than 100 amino acid residues. ORF biC834 shows 100% bp identity with the recently identified multicopy suppressor gene of the pop2 mutation (MPT5); its deduced protein product carries an eight-repeat domain region, homologous to that found in the hypothetical regulatory YGL023 protein of S. cerevisiae and the Pumilio protein of Drosophila. ORF biE560 protein exhibits patterns typical of serine/threonine protein kinases, with which it shares high degrees of homology.

摘要

我们报道了来自酿酒酵母七号染色体左臂的一段7941 bp DNA片段的序列,该片段包含四个氨基酸残基超过100个的开放阅读框(ORF)。ORF biC834与最近鉴定出的pop2突变多拷贝抑制基因(MPT5)具有100%的碱基同一性;其推导的蛋白质产物带有一个八重复结构域区域,与酿酒酵母假设的调控蛋白YGL023和果蝇的Pumilio蛋白中的结构域同源。ORF biE560蛋白表现出丝氨酸/苏氨酸蛋白激酶的典型模式,与之具有高度同源性。

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