Bou G, Esteban P F, Baladron V, Gonzalez G A, Cantalejo J G, Remacha M, Jimenez A, Del Rey F, Ballesta J P, Revuelta J L
Centro de Biología Molecular, CSIC and UAM, Canto Blanco, Madrid, Spain.
Yeast. 1993 Dec;9(12):1349-54. doi: 10.1002/yea.320091209.
As part of the EEC yeast genome program, a fragment of 15,820 bp from the right arm of Saccharomyces cerevisiae chromosome XI has been sequenced. This fragment corresponds roughly to the centromere-distal half of cosmid pUKG046 and to a small fragment of cosmid pUKG096, which are located approximately 150 kb from the centromere. It contains four open reading frames (ORFs) which encode potential proteins of more than 100 amino acid residues, as well as the UBI2 gene which carries an intron and does not show up as an ORF in the sequence analysis programs. One of the putative proteins, YKR412, is very rich in serine and has significant homology at the carboxyl end to Nopp140 phosphoprotein. YKR413 has several predicted transmembrane domains. YKR15, which has been recently cloned as the MPL1 gene, encodes a polypeptide that shows homologies to myosin heavy chain and to the cytoskeleton protein Uso1.
作为欧洲经济共同体酵母基因组计划的一部分，已对来自酿酒酵母第十一染色体右臂的一段15,820 bp的片段进行了测序。该片段大致对应于黏粒pUKG046着丝粒远端的一半以及黏粒pUKG096的一小段，它们位于距离着丝粒约150 kb处。它包含四个开放阅读框（ORF），这些开放阅读框编码潜在的超过100个氨基酸残基的蛋白质，以及携带一个内含子且在序列分析程序中未显示为开放阅读框的UBI2基因。其中一个推定的蛋白质YKR412富含丝氨酸，并且在羧基末端与Nopp140磷蛋白具有显著的同源性。YKR413有几个预测的跨膜结构域。最近作为MPL1基因克隆的YKR15编码一种与肌球蛋白重链和细胞骨架蛋白Uso1具有同源性的多肽。
