Hu J H, Sun D X, Zeng G Q, Lin A Y, Rui Y C
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China.
Zhongguo Yao Li Xue Bao. 1995 Jul;16(4):318-21.
To study platelet activating factor (PAF) stimulating the platelets to adhere to cultured bovine cerebral microvascular endothelial cells (CMEC) and the inhibitory effect of triazelodiazepine (WEB), 1,5-bis-(3,4-dimethoxyphenyl)-tetrahydro-(4H)-pyran (DMPP), tetrandrine (Tet).
The platelets adhesion to CMEC and the inhibitory effect of drugs were investigated by [3H]adenine labeling of rabbit blood platelet.
The platelet adhesion to CMEC was increased by 36% vs control after CMEC was stimulated with PAF 10 nmol L-1 for 25 min. WEB 0.1, 1, 10 mmol L-1 or DMPP 0.1, 1, 10 mmol L-1 or Tet 0.1, 1, 10 mmol L-1 inhibited the PAF stimulating platelet adhesion to CMEC by 5.4%, 16.3%, 20.1%; 13.7%, 19.4%, 22.4%; and 5.5%, 23.1%, 32.6%, respectively.
DMPP and Tet inhibited the PAF action in cerebral vascular system.
研究血小板活化因子（PAF）刺激血小板黏附于培养的牛脑微血管内皮细胞（CMEC）的作用以及三氮唑二氮䓬（WEB）、1,5-双-（3,4-二甲氧基苯基）-四氢-（4H）-吡喃（DMPP）、粉防己碱（Tet）的抑制作用。
采用[3H]腺嘌呤标记兔血小板，研究血小板对CMEC的黏附作用及药物的抑制作用。
用10 nmol/L PAF刺激CMEC 25分钟后，血小板对CMEC的黏附较对照组增加了36%。0.1、1、10 mmol/L的WEB或0.1、1、10 mmol/L的DMPP或0.1、1、10 mmol/L的Tet分别抑制PAF刺激的血小板对CMEC的黏附达5.4%、16.3%、20.1%；13.7%、19.4%、22.4%；以及5.5%、23.1%、32.6%。
DMPP和Tet在脑血管系统中抑制PAF的作用。
