通过血小板活化因子刺激及药物拮抗作用使血小板黏附于培养的牛脑微血管内皮细胞
Platelet adhesion to cultured bovine cerebral microvascular endothelial cells by stimulation of platelet activating factor and antagonism of drugs.
作者信息
Hu J H, Sun D X, Zeng G Q, Lin A Y, Rui Y C
机构信息
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China.
出版信息
Zhongguo Yao Li Xue Bao. 1995 Jul;16(4):318-21.
AIM
To study platelet activating factor (PAF) stimulating the platelets to adhere to cultured bovine cerebral microvascular endothelial cells (CMEC) and the inhibitory effect of triazelodiazepine (WEB), 1,5-bis-(3,4-dimethoxyphenyl)-tetrahydro-(4H)-pyran (DMPP), tetrandrine (Tet).
METHODS
The platelets adhesion to CMEC and the inhibitory effect of drugs were investigated by [3H]adenine labeling of rabbit blood platelet.
RESULTS
The platelet adhesion to CMEC was increased by 36% vs control after CMEC was stimulated with PAF 10 nmol L-1 for 25 min. WEB 0.1, 1, 10 mmol L-1 or DMPP 0.1, 1, 10 mmol L-1 or Tet 0.1, 1, 10 mmol L-1 inhibited the PAF stimulating platelet adhesion to CMEC by 5.4%, 16.3%, 20.1%; 13.7%, 19.4%, 22.4%; and 5.5%, 23.1%, 32.6%, respectively.
CONCLUSION
DMPP and Tet inhibited the PAF action in cerebral vascular system.
目的
研究血小板活化因子（PAF）刺激血小板黏附于培养的牛脑微血管内皮细胞（CMEC）的作用以及三氮唑二氮䓬（WEB）、1,5-双-（3,4-二甲氧基苯基）-四氢-（4H）-吡喃（DMPP）、粉防己碱（Tet）的抑制作用。
方法
采用[3H]腺嘌呤标记兔血小板，研究血小板对CMEC的黏附作用及药物的抑制作用。
结果
用10 nmol/L PAF刺激CMEC 25分钟后，血小板对CMEC的黏附较对照组增加了36%。0.1、1、10 mmol/L的WEB或0.1、1、10 mmol/L的DMPP或0.1、1、10 mmol/L的Tet分别抑制PAF刺激的血小板对CMEC的黏附达5.4%、16.3%、20.1%；13.7%、19.4%、22.4%；以及5.5%、23.1%、32.6%。
结论
DMPP和Tet在脑血管系统中抑制PAF的作用。
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