利用白细胞迁移抑制试验评估人类乳腺癌和黑色素瘤中的抗原差异。

Use of the leukocyte migration inhibition assay to evaluate antigenic differences in human breast cancers and melanomas.

作者信息

Cannon B, McCoy J L, Connor R J, Jerome L, Keys L, Dean H H, Herberman R B

出版信息

J Natl Cancer Inst. 1978 May;60(5):969-78. doi: 10.1093/jnci/60.5.969.

DOI:10.1093/jnci/60.5.969

Abstract

The leukocyte migration inhibition assay was used to compare the antigenic reactivity of 3 M KCl extracts of human tumors. Many extracts demonstrated strong reactivity with patient leukocytes, whereas others demonstrated weak or no reactivity, Extracts prepared from primary tumors or local recurrent tumors were more antigenic than extracts from involved lymph nodes or pleural effusions. The least reactive preparations were extracts made from specimens of liver metastases obtained at autopsy. A large standard extract tested at a standard concentration was useful for the evaluation of antigenic reactivity of human tumor extracts. It served as a point of reference in simultaneous tests with one blood sample from each individual, thus eliminating the influence of patient variation on extract reactivity.

摘要

采用白细胞迁移抑制试验比较人肿瘤3M氯化钾提取物的抗原反应性。许多提取物与患者白细胞表现出强反应性，而其他提取物则表现出弱反应性或无反应性。原发性肿瘤或局部复发性肿瘤制备的提取物比受累淋巴结或胸腔积液的提取物更具抗原性。反应性最低的制剂是尸检时获得的肝转移标本提取物。以标准浓度测试的大标准提取物有助于评估人肿瘤提取物的抗原反应性。它在对每个个体的一份血样进行同步检测时作为参考点，从而消除患者个体差异对提取物反应性的影响。