Cooper J F, Foti A G, Shank P W
J Urol. 1978 Mar;119(3):392-5. doi: 10.1016/s0022-5347(17)57499-6.
Human acid phosphatases are ubiquitous phosphohydrolases that are present in most respiring tissues and cells. Specifically, human prostatic acid phosphatase is a unique enzyme within a vast family of acid phosphatases concerned with catabolic processes in cellular metabolism. The majority of serum and bone marrow acid phosphatases are of non-prostatic origin and are present chiefly in erythrocytes, leukocytes, platelets and other maturing cells in the bone marrow. The specific concentration of prostatic acid phosphatase in serum and bone marrow is normally relatively low compared to non-prostatic acid phosphatases. Many falsely positive assays for total serum acid phosphatases and bone marrow acid phosphatases have been reported, particularly after traumatic marrow biopsy procedures and mishandling of blood samples in the clinical laboratory and in hematologic disease states. The disruption and lysis of whole blood and marrow cells can liberate non-specific acid phosphatases into the serum. Since standard enzymatic assays do not discriminate accurately prostatic acid phosphatase from non-prostatic acid phosphatase present in the serum spurious results can be realized. A preliminary experience with a promising radioimmunoassay for the specific measurement of prostatic acid phosphatase in bone marrow and serum is presented.
人酸性磷酸酶是普遍存在的磷酸水解酶，存在于大多数进行呼吸作用的组织和细胞中。具体而言，人前列腺酸性磷酸酶是庞大酸性磷酸酶家族中的一种独特酶，与细胞代谢中的分解代谢过程有关。大多数血清和骨髓酸性磷酸酶并非源自前列腺，主要存在于红细胞、白细胞、血小板及骨髓中的其他成熟细胞中。与非前列腺酸性磷酸酶相比，血清和骨髓中前列腺酸性磷酸酶的特定浓度通常相对较低。已有许多关于血清总酸性磷酸酶和骨髓酸性磷酸酶的假阳性检测结果的报道，特别是在创伤性骨髓活检操作后，以及临床实验室和血液疾病状态下血液样本处理不当的情况下。全血和骨髓细胞的破坏与裂解可使非特异性酸性磷酸酶释放到血清中。由于标准酶法检测无法准确区分血清中前列腺酸性磷酸酶和非前列腺酸性磷酸酶，可能会得出虚假结果。本文介绍了一种有前景的放射免疫分析法用于特异性检测骨髓和血清中前列腺酸性磷酸酶的初步经验。
