George J, Byth K, Farrell G C
Department of Gastroenterology and Hepatology, University of Sydney, Westmead Hospital, NSW, Australia.
Biochem Pharmacol. 1995 Aug 25;50(5):727-30. doi: 10.1016/0006-2952(95)00192-3.
Multivariate linear regression analysis was used to examine the influence of age, gender and environmental variables on the hepatic content of cytochromes P450 (CYP, P450) 1A2, 2C, 2E1 and 3A in 71 subjects; 21 with histologically normal livers and 50 with chronic liver disease. There was a clear negative association between age and total P450 content, NADPH-cytochrome c reductase activity and levels of 2E1 and 3A proteins. 1A2 and 2C proteins were unaltered with advancing age. Gender did not influence the expression of any of the CYP proteins. Cigarette smoking was associated with enhanced levels of 1A2, but effects of drug ingestion and alcohol consumption were not apparent in this study, probably because of case selection. It is concluded that age but not gender is a constitutional factor that influences the hepatic content of cytochrome P450 and selected CYP proteins.
采用多元线性回归分析，研究年龄、性别和环境变量对71名受试者肝脏中细胞色素P450（CYP，P450）1A2、2C、2E1和3A含量的影响；其中21名肝脏组织学正常，50名患有慢性肝病。年龄与总P450含量、NADPH - 细胞色素c还原酶活性以及2E1和3A蛋白水平之间存在明显的负相关。随着年龄增长，1A2和2C蛋白未发生改变。性别不影响任何CYP蛋白的表达。吸烟与1A2水平升高有关，但本研究中药物摄入和饮酒的影响不明显，可能是由于病例选择的原因。结论是年龄而非性别是影响细胞色素P450和特定CYP蛋白肝脏含量的体质因素。
