Butler R G
Can J Microbiol. 1975 Dec;21(12):2089-93. doi: 10.1139/m75-298.
A flow-through dialysis system used to decrease the concentrations of toxic organic materials excreted by Thiobacillus thiooxidans permitted an improved efficiency of carbon dioxide fixation when compared with cells taken from the usual shaken culture. The additions of various concentrations of pyruvic acid and succinic acid inhibited growth significantly. Pyruvate at a concentration of 5 X 10(-3) M completely inhibited the respiration of resting cells oxidizing sulfur. The toxicity of pyruvic acid was found to be permanent as evidenced by the inability to obtain satisfactory oxidation rates after washing the exposed cells twice in buffer. Both pyruvate (10(-3) M) and succinate (10(-3) M) inhibited carbon dioxide fixation by 84%.
与从常规振荡培养物中获取的细胞相比，用于降低硫氧化硫杆菌排泄的有毒有机物质浓度的流通式透析系统可提高二氧化碳固定效率。添加不同浓度的丙酮酸和琥珀酸会显著抑制生长。浓度为5×10⁻³ M的丙酮酸完全抑制了静止细胞氧化硫的呼吸作用。丙酮酸的毒性被发现是永久性的，这可通过在缓冲液中洗涤暴露的细胞两次后仍无法获得令人满意的氧化速率来证明。丙酮酸（10⁻³ M）和琥珀酸（10⁻³ M）均使二氧化碳固定率降低84%。
