Rao G S, Berger L R
J Bacteriol. 1970 May;102(2):462-6. doi: 10.1128/jb.102.2.462-466.1970.
Addition of 10(-3)m pyruvic acid to cultures of Thiobacillus thiooxidans, at pH 2.3, results in its rapid intracellular accumulation and in the cessation of sulfur oxidation, CO(2) fixation, and oxygen consumption; at pH 7.0, pyruvate neither inhibits oxygen uptake nor accumulates appreciably intracellularly. Pyruvate does not affect CO(2) fixation in cell-free extracts. The data suggest that the cells of T. thiooxidans are passively permeable to pyruvic acid at low pH. Thus entry of pyruvic acid causes accumulation of pyruvate with a concomitant decrease in intracellular pH.
在pH 2.3条件下，向氧化硫硫杆菌培养物中添加10⁻³m丙酮酸，会导致其在细胞内迅速积累，并使硫氧化、二氧化碳固定和氧气消耗停止；在pH 7.0时，丙酮酸既不抑制氧气摄取，也不会在细胞内明显积累。丙酮酸不影响无细胞提取物中的二氧化碳固定。数据表明，在低pH条件下，氧化硫硫杆菌细胞对丙酮酸是被动通透的。因此，丙酮酸的进入会导致丙酮酸积累，同时细胞内pH值下降。
