Ohtsuka E, Kikuchi H, Abe Y, Moriyama K, Ohno E, Hirota K, Tezono K, Nasu M
Second Department of Internal Medicine, Oita Medical University, Japan.
Br J Haematol. 1995 Aug;90(4):951-3. doi: 10.1111/j.1365-2141.1995.tb05223.x.
We report a patient who developed Philadelphia chromosome negative acute myeloblastic leukaemia with trisomy 8 and trisomy 11 after receiving treatment with alkylating agents and interferon for chronic myelocytic leukaemia positive for Philadelphia chromosome. Leukaemic cells were positive for myeloperoxidase and expressed CD13, CD33 and DR; some expressed CD2, CD4 and CD34. The fluorescence in situ hybridization method revealed that bcr-abl fusion genes were absent from > 90% of the bone marrow cells. The major bcr rearrangement was not detected by Southern blot analysis. We conclude that the leukaemic cells negative for Philadelphia chromosome may have developed as a result of treatment with alkylating agents and interferon in the present case.
我们报告了一名患者，该患者在接受烷化剂和干扰素治疗费城染色体阳性的慢性粒细胞白血病后，发生了伴有8号染色体三体和11号染色体三体的费城染色体阴性急性髓细胞白血病。白血病细胞髓过氧化物酶呈阳性，表达CD13、CD33和DR；部分表达CD2、CD4和CD34。荧光原位杂交法显示，超过90%的骨髓细胞中不存在bcr-abl融合基因。Southern印迹分析未检测到主要的bcr重排。我们得出结论，在本病例中，费城染色体阴性的白血病细胞可能是烷化剂和干扰素治疗的结果。
