Schubert F R, Fainsod A, Gruenbaum Y, Gruss P
Abteilung für Molekulare Zellbiologie, Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany.
Mech Dev. 1995 May;51(1):99-114. doi: 10.1016/0925-4773(95)00358-8.
We have isolated the novel murine Sax-1 gene, a member of the NK-1 class of homeobox genes, and report its expression pattern in the developing central nervous system (CNS) in comparison to two other homeobox genes, Evx-1 and Pax-6. Sax-1 was found to be transiently expressed in the developing posterior CNS. First seen in the ectoderm lateral to the primitive streak, the signal later encompassed the neural plate. Posteriorly, the expression domain overlapped with the Evx-1 expression in the streak, while anteriorly it was delimited by the Pax-6 signal in the neural tube. This early phase starting at day 9.5 pc, Sax-1 was expressed in distinct areas of spinal cord, hindbrain and forebrain. Particularly strong signals were detected in rhombomere 1 and in the pretectum. In these areas, subsets of neurons may be marked and specified. In addition to the normal pattern of Sax-1 during development, the expression in different mouse mutants was analysed. In Brachyury curtailed homozygotes, the expression of Sax-1 was found to be reduced during neurulation and even lost at day 9.0 pc. Ventral shift and finally loss of the signal in the ventral spinal cord was observed in Danforth's short tail homozygotes.
我们分离出了新型小鼠Sax-1基因，它是同源异型盒基因NK-1家族的成员，并报告了其在发育中的中枢神经系统（CNS）中的表达模式，同时与另外两个同源异型盒基因Evx-1和Pax-6进行了比较。发现Sax-1在发育中的中枢神经系统后部短暂表达。最初在原条外侧的外胚层中可见，该信号随后覆盖神经板。在后部，表达域与原条中Evx-1的表达重叠，而在前部则由神经管中的Pax-6信号界定。在胚胎第9.5天开始的这个早期阶段，Sax-1在脊髓、后脑和前脑的不同区域表达。在菱脑节1和顶盖前区检测到特别强的信号。在这些区域，可能标记并指定了神经元亚群。除了发育过程中Sax-1的正常模式外，还分析了其在不同小鼠突变体中的表达。在短尾纯合子中，发现Sax-1在神经胚形成期间表达减少，甚至在胚胎第9.0天消失。在丹佛短尾纯合子中，观察到脊髓腹侧信号发生腹侧移位并最终消失。
