Landry C, Clotman F, Hioki T, Oda H, Picard J J, Lemaigre F P, Rousseau G G
Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Dev Biol. 1997 Dec 15;192(2):247-57. doi: 10.1006/dbio.1997.8757.
Hepatocyte nuclear factor-6 (HNF-6) is a liver-enriched transcription factor that contains a single cut domain and a novel type of homeodomain. Here we have studied the developmental expression pattern of HNF-6 in the mouse. In situ hybridization experiments showed that HNF-6 mRNA is detected in the liver at embryonic day (E) 9, at the onset of liver differentiation. HNF-6 mRNA disappeared transiently from the liver between E12.5 and E15. In transfection experiments HNF-6 stimulated the expression of HNF-4 and of HNF-3 beta, two transcription factors known to be involved in liver development and differentiation. HNF-6 was detected in the pancreas from E10.5 onward, where it was restricted to the exocrine cells. HNF-6 was also detected in the developing nervous system. Both the brain and the spinal cord started to express HNF-6 at E9-9.5 in postmitotic neuroblasts. Later on, HNF-6 was restricted to brain nuclei, to the retina, to the ventral horn of the spinal cord, and to dorsal root ganglia. Our observations that HNF-6 contributes to the control of the expression of transcription factors and is expressed at early stages of liver, pancreas, and neuronal differentiation suggest that HNF-6 regulates several developmental programs.
肝细胞核因子6（HNF - 6）是一种肝脏富集转录因子，它包含一个单一的剪切结构域和一种新型的同源结构域。在此，我们研究了HNF - 6在小鼠体内的发育表达模式。原位杂交实验表明，在胚胎第9天（E9）肝脏分化开始时即可检测到HNF - 6 mRNA。在E12.5至E15期间，HNF - 6 mRNA在肝脏中短暂消失。在转染实验中，HNF - 6刺激了HNF - 4和HNF - 3β的表达，这两种转录因子已知参与肝脏发育和分化。从E10.5开始在胰腺中检测到HNF - 6，它局限于外分泌细胞。在发育中的神经系统中也检测到了HNF - 6。在有丝分裂后的神经母细胞中，大脑和脊髓在E9 - 9.5时开始表达HNF - 6。之后，HNF - 6局限于脑细胞核、视网膜、脊髓腹角和背根神经节。我们观察到HNF - 6有助于控制转录因子的表达，并且在肝脏、胰腺和神经元分化的早期阶段表达，这表明HNF - 6调节多个发育程序。
