Kaneko S, Kaneko M, Ohkawa H
Department of Pediatric Surgery, University of Tsukuba.
Hum Cell. 1995 Mar;8(1):35-41.
A childhood embryonal rhabdomyosarcoma cell line, designated as TS-RM-1, was established from transplanted tumor in nude mouse. TS-RM-1 cells were small, spindle to polygonal shaped and cytoplasm was rich in glycogen. Estimated population doubling time was 31 hours and the distribution of chromosome number was in the range of 88 to 98. The karyotype of TS-RM-1 cells revealed nonrandom structural chromosome alterations, including der(3)t(1;3)(q12;p12-14),16q-,17q+ and 21q+. In immuno-cytohistochemical study, both TS-RM-1 cells and the primary tumor were positive for desmin and vimentin. TS-RM-1 cell line may be useful for studying the association between embryonal rhabdomyosarcoma and a specific alteration in chromosome 3.
一种儿童胚胎性横纹肌肉瘤细胞系，命名为TS-RM-1，是从裸鼠移植瘤中建立的。TS-RM-1细胞较小，呈梭形至多边形，细胞质富含糖原。估计群体倍增时间为31小时，染色体数目分布在88至98之间。TS-RM-1细胞的核型显示出非随机的染色体结构改变，包括der(3)t(1;3)(q12;p12-14)、16q-、17q+和21q+。在免疫细胞化学研究中，TS-RM-1细胞和原发肿瘤的结蛋白和波形蛋白均呈阳性。TS-RM-1细胞系可能有助于研究胚胎性横纹肌肉瘤与3号染色体特定改变之间的关联。
