Hamdan M, Curcuruto O, Di Modugno E
Glaxo Research Laboratories, Verona, Italy.
Rapid Commun Mass Spectrom. 1995;9(10):883-7. doi: 10.1002/rcm.1290091004.
Complexation in aqueous solutions between vancomycin, ristocetin A, teicoplanin and two bacterial cell-wall analogues, Ac2-L-Lys-D-Ala-D-Ala (tripeptide) and Ac-D-Ala-D-Ala (dipeptide) has been examined by positive-ion electrospray mass spectrometry (ES-MS) and capillary zone electrophoresis (CZE)/ES-MS. The ES-MS data demonstrate that as well as complexes between monomeric antibiotics and either peptide, the investigated solutions contained complexes ranging from the simple homodimer of the antibiotic to a more complex association giving ions of the type [2(antibiotic) + 3(tripeptide) + 3H]3+. The same data also demonstrate that the homodimers of the investigated antibiotics are significantly suppressed in the presence of the tripeptide. The use of CZE/ES-MS made it evident that the complexes between the antibiotic and the tripeptide were present in the solution prior to their introduction into the ion source. The two sets of data are compared with existing UV difference spectroscopy and nuclear magnetic resonance data on complexation and dimerization.
通过正离子电喷雾质谱（ES-MS）和毛细管区带电泳（CZE）/ES-MS研究了万古霉素、瑞斯托菌素A、替考拉宁与两种细菌细胞壁类似物Ac2-L-Lys-D-Ala-D-Ala（三肽）和Ac-D-Ala-D-Ala（二肽）在水溶液中的络合情况。ES-MS数据表明，除了单体抗生素与任一肽之间的络合物外，所研究的溶液中还含有从抗生素的简单同二聚体到更复杂缔合物的络合物，产生了[2(抗生素)+3(三肽)+3H]3+类型的离子。相同的数据还表明，在所研究的抗生素的同二聚体在三肽存在下会受到显著抑制。CZE/ES-MS的使用表明，抗生素与三肽之间的络合物在引入离子源之前就已存在于溶液中。将这两组数据与现有的关于络合和二聚化的紫外差光谱和核磁共振数据进行了比较。
